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Use of a Fluorophore to Tag Arthropods for Mark-Release-Recapture Type Research
Authors:James R Hagler  Allya M Hull  Miles T Casey  Scott A Machtley
Institution:USDA-ARS, Arid-Land Agricultural Research Center, 21882 North Cardon Lane, Maricopa, AZ 85138, USA
Abstract:We examined the feasibility of externally marking insects with the liquid fluorescent forensic theft deterrent, SmartWater (SmartWater CSI, LLC.). We sprayed captive Lygus hesperus (Knight) (Hemiptera: Miridae), Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), and Hippodamia convergens Guérin-Méneville (Coleoptera: Coccinellidae) with SmartWater fluorophore, and then qualitatively examined them for fluorescence by visual inspection under ultraviolet (UV) light and quantitatively measured them with a multiwavelength microplate fluorometer. The results indicate that this product has enormous potential as a taggant for L. hesperus and B. tabaci. However, the marking efficiency for H. convergens was only adequate. The advantages and limitations of using SmartWater as a biological marker for arthropod mark-release-recapture research are discussed.
Keywords:fluorescent mark  dispersal  central point release  Bemisia tabaci  Lygus hesperus
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