紫红薯糖蛋白体外抗氧化活性研究

研究紫红薯糖蛋白的体外抗氧化活性。使用水提醇沉方法、Sevage法除游离蛋白,用DEAE-52纤维素层析及SephadexG-75纯化制备糖蛋白。采用超氧阴离子自由基(O-2.)、羟基自由基(·OH)、1,1-二苯基-2-苯肼自由基(DPPH.)清除率测定法及还原力测定法评价紫红薯糖蛋白体外抗氧化活性。糖蛋白在0～640μg/mL内,均具有明显的抗氧化活性,随着质量浓度的增加活性增强;其中在紫红薯糖蛋白的质量浓度为640μg/mL时,对超氧阴离子自由基(O-2.)清除作用达到69.80%;对羟基自由基(.OH)的清除作用达到73.29%。

Antioxidant Activity of Glycoprotein from the Purple Sweet Potato

To study the anti-oxidant activity of glycoprotein from purple sweet potato.The glycoprotein from the purple sweet potato was extracted by water extracting-alcohol precipitating process,and the protein was removed by Sevage method.Then the glycoprotein was isolated and purified by cellulose column and Sephadex G-75.The antioxidant activity of purple sweet potato glycoprotein was evaluated by superoxide anion free radical(O-2·),hydroxyl free radical(·OH),1,1-diphenyl-2-phenylhydrazine radical(DPPH·) and reductive force.The glycoprotein powder has significant antioxidant activity in the range of 0-640 μg/mL concentration,and the activity was enhanced with increased concentration.when the concentration of glycoprotein was 640 μg/mL,the clearance effect on superoxide anion free radical(O-2·)could reach 69.80%,the clearance effect on hydroxyl free radical(·OH) could reach 73.29%.