Genetic structure composed of additive QTL,epistatic QTL pairs and collective unmapped minor QTL conferring oil content and fatty acid components of soybeans |
| |
Authors: | Haiwang?Li Tuanjie?Zhao Yufeng?Wang Deyue?Yu Shouyi?Chen Ruibao?Zhou Email author" target="_blank">Junyi?GaiEmail author |
| |
Institution: | (1) Soybean Research Institute, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China;(2) National Center for Soybean Improvement, Nanjing, 210095, Jiangsu, People’s Republic of China;(3) National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing, 210095, Jiangsu, People’s Republic of China;(4) Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China;(5) Soybean Processing Research Institute, Henan University of Technology, Zhengzhou, 450052, Henan, People’s Republic of China; |
| |
Abstract: | The relative importance of various types of quantitative trait locus (QTL) conferring oil content and its fatty acid components
in soybean seeds was assessed through testing a recombinant inbred line (RIL) population (derived from KF1 × NN1138-2) in
randomized blocks experiments in 2004–2006. The contents of oil and oleic, linoleic, linolenic, palmitic and stearic acids
were determined with automatic Soxhlet extraction system and gas chromatography, respectively. Based on the established genetic
linkage map with 834 markers, QTLNetwork2.0 was used to detect QTL under the genetic model composed of additive, additive × additive
(epistasis), additive × year and epistasis × year effects. The contributions to the phenotypic variances of additive QTL and
epistatic QTL pairs were 15.7% (3 QTL) and 10.8% (2 pairs) for oil content, 10.4% (3 QTL) and 10.3% (3 pairs) for oleic acid,
11.6% (3 QTL) and 8.5% (2 pairs) for linoleic acid, 28.5% (7 QTL) and 7.6% (3 pairs) for linolenic acid, 27.0% (6 QTL) and
16.6% (7 pairs) for palmitic acid and 29.7% (5 QTL) and 4.3% (1 pair) for stearic acid, respectively. Those of additive QTL
by year interaction were small and no epistatic QTL pair by year interaction was found. Among the 27 additive QTL and 36 epistatic
QTL (18 pairs), three are duplicated between the two QTL types. A large difference was found between the genotypic variance
among RILs and the total variance of mapped QTL, which accounted for 52.9–74.8% of the genotypic variation, much larger than
those of additive QTL and epistatic QTL pairs. This part of variance was recognized as that due to a collection of unmapped
minor QTL, like polygenes in biometrical genetics, and was designated as collective unmapped minor QTL. The results challenge
the breeders for how to pyramid different types of QTL. In addition, the present study supports the mapping strategy of a
full model scanning followed by verification with other procedures corresponding to the first results. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|