Harnessing mutant donor plants for microspore culture in Indian mustard [Brassica juncea (L.) Czern and Coss] |
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Authors: | Deepak Prem Kadambari Gupta Abha Agnihotri |
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Institution: | (1) Plant Development and Nuclear Organization Unit, Centro de Investigaciones Biol?gicas, Biological Research Centre, (CIB) C.S.I.C., Ramiro de Maeztu 9, 28040 Madrid, Spain;(2) Topworth Group of Companies, A–21, 2nd Floor, Green Park Main, New Delhi, 110016, India;(3) Amity Institute of Microbial Technology, Amity University, Block E-3, 4th Floor, Sector 125, Noida, 201303, Uttar Pradesh, India |
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Abstract: | Haploid mutagenesis offers several advantages over conventional (seed) approach. However, its potential has not been utilised
for Brassica juncea, an important oilseed. In this study, mutant donor plants of three Indian B. juncea genotypes, generated by ethyl methanesulfonate (EMS) and ethyl nitrosourea (ENU), were used for microspore culture. The response
of mutant donor plants was about 100 times lower than non-mutant controls; a total of 9,411 embryos were produced from the
EMS treated donor plants, while microspores isolated from ENU treated donors did not yield any embryos. The lethality of induced
mutations demonstrated itself mainly as the induction of abnormal embryos (80%), failure of germination (70%) and failure
of plantlet development (70%). Nine doubled haploid (DH) mutant lines and three non-mutant DH lines obtained through this
approach were tested for agronomic and biochemical variation over two growing seasons. High variability was observed and stable
mutants were recovered for reduced height (125 vs. 168 cm for the control), appressed pod character, altered fatty acid composition
higher protein proportion in de-oiled meal (48%) and a lower glucosinolate content in de-oiled meal (59.5 μM/g) relative to
controls. The approach demonstrates that despite severe reduction in efficiency of the DH line production, valuable mutants
can be recovered from mutated donor plants. |
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