Production and characterization of amphihaploid hybrids between <Emphasis Type="Italic">Nicotiana wuttkei</Emphasis> Clarkson et Symon and <Emphasis Type="Italic">N. tabacum</Emphasis> L |
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Authors: | Dorota Laskowska Apoloniusz Berbe? |
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Institution: | (1) Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation-State Research Institute, ul. Czartoryskich 8, 24-100 Puławy, Poland |
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Abstract: | Nicotiana wuttkei Clarkson and Symon discovered in the 1990s in Australia may be of potential interest to breeders as it carries resistance
to Peronospora hyoscyami de Bary. The crossability between N. wuttkei (2n = 4x = 32) and three N. tabacum (2n = 4x = 48) cultivars (‘Puławski 66’, ‘Wiślica’ and ‘TN 90’) and the morphology and cytology of their amphihaploid hybrids (2n = 4x = 40) were studied. Seeds were produced only when N. wuttkei was used as the maternal parent, but under normal germination all seedlings died. Viable F1 hybrids of N. wuttkei × N. tabacum cv. ‘Puławski’ and N. wuttkei × N. tabacum cv. ‘Wiślica’ were obtained only by in vitro cotyledon culture. The amphihaploid plants were intermediate between the parents
for most morphological traits. In 46.4% of the PMC’s, only univalents were present. The remainder of the cells had 1–5 bivalents
and 1–2 trivalents. In spite of a detectable frequency of monads (2.6%), dyads (2.6%) and triads (4.5%), the hybrids were
self and cross sterile. |
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