<Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated transformation of cotton (<Emphasis Type="Italic">Gossypium hirsutum</Emphasis> L.) with a fungal phytase gene improves phosphorus acquisition |
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Authors: | J F Liu C Y Zhao J Ma G Y Zhang M G Li G J Yan X F Wang Z Y Ma |
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Institution: | (1) North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, Baoding, 071001, China;(2) Institute for Molecular Biology, Nankai University, Tianjin, 300071, China;(3) School of Plant Biology, The University of Western Australia, Crawley, WA, 6009, Australia; |
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Abstract: | A phytase gene (phyA), isolated from Aspergillus ficuum (AF537344), was introduced into cotton (Gossypium hirsutum L.) by Agrobacterium-mediated transformation to increase the phosphorus (P) acquisition efficiency of cotton. Southern and Northern blot analyses
showed that the phyA was successfully incorporated into the cotton genome and expressed in transgenic lines. After growing for 45 days with phytate
(Po) as the only P source, the shoot and root dry weights of the transgenic plants all increased by nearly 2.0-fold relative
to those of wild-type plants, but were similar to those of transgenic plants supplied with inorganic phosphorus. The phytase
activities of root extracts prepared from transgenic plants were 2.4- to 3.6-fold higher than those from wild-type plants,
and the extracellular phytase activities of transgenic plants were also 4.2- to 6.3-fold higher. Furthermore, the expressed
phytase was secreted into the rhizospheres as demonstrated by enzyme activity staining. The transgenic plants accumulated
much higher contents of total P (up to 2.1-fold after 30 days of growth) than the wild-type plants when supplied with Po.
These findings clearly showed that cotton plant transformed with a fungal phytase gene was able to secret the enzyme from
the root, which markedly improved the plant’s ability to utilize P from phytate. This may serve as a promising step toward
the development of new cotton cultivars with improved phosphorus acquisition. |
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