我国主要棉花栽培群体的遗传多样性分析

摘 要：利用ISSR分子标记对我国44个棉花栽培品种（拟似为6个群体）进行遗传多样性分析。筛选出的11个引物在所有品种中共扩增出90条带，其中70条为多态性条带，多态性条带百分率(PPB)达76.1%，Popgene32软件分析结果表明，新疆种群的遗传多样性水平最高(PPL=91.43%，H=0.3769，I=0.5464)，其次是中棉种群、河南种群，而河北、山东、山西3个种群的遗传多样性水平相差较小，6个棉花种群在其总的遗传变异中有82.42%的存在于群体内，群体间的遗传变异仅占总变异的17.58%，群体分化系数Gst=0.1759，群体间总的基因流的估算值Nm=2.34。由聚类分析可知，山西、山东、河北种群的亲缘关系最近，先聚合在一起，然后依次与河南、中棉所种群聚类，最后与亲缘关系最远的新疆种群聚合。

Genetic Diversity Analysis on the Main Chinese Cultivated Varieties of 6 Cotton Populations with ISSR Markers

Abstract: In the present paper, the genetic diversity 6 kind of 48 cotton trains from different origins were estimated using inter simple sequence repeat (ISSR) markers. 11 among 60 ISSR primers were chosen for their reproducibility and high polymorphism. a total of 90 fragments were amplified, in which the percentage of polymorphic loci (PPL) was76.1%, Results of POPGENE analysis indicated that genetic diversity of Xinjiang population was the highest (PPB= 91.43%, H= 0.3769, I= 0.5464),and Zhongmiansuo, Henan population followed it and genetic diversity was near among other three population. Analysis of Nei’s genetic diversity index indicated that the intraspecific genetic diversity were 82.42% ,diversity among populations were 19.72%; the gene differentiation (Gst) was 0.1759 and the gene flow (Nm) was 2.34 ;Result of UPGMA cluster analysis indicated that the relationship between Shandong and Shanxi is nearest It were the first clustered with Hebei, and Henan and CCAS were followed successively, Xinjiang population were clustered finally.