Intertribal somatic hybridization between rapid cycling Brassica oleracea L. and Camelina sativa (L.) Crantz |
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Authors: | Lise N Hansen |
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Institution: | (1) Dept. of Agricultural Sciences, Section Plant Breeding and Crop Science, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark |
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Abstract: | Black spot, caused by Alternaria brassicae and A. brassicicola, is an important disease in all Brassica oleracea vegetables.
Sufficient resistance to the pathogen is not found within the species, nor in species that readily cross to B. oleracea. Camelina
sativa (false flax) is highly resistant to Alternaria spp. and has, in addition, other desirable characters for the improvement
of B. oleracea. Protoplast fusions were performed between rapid cycling B. oleracea (tribe Brassiceae), which has good regenerability,
and C. sativa (tribe Sisymbrieae) by polyethylene glycol (PEG) treatment. The B. oleracea fusion partner was inactivated by
treatment with iodoacetate. C. sativa has poor regenerability; hence, no pretreatment was needed for this species. The protoplasts
were cultured using a feeder layer system. A total of 2903 calli were isolated from the fusions. Fourteen of these initiated
shoots, i.e., 0.5% regeneration frequency. Approximately 110 shoots were excised from 6 of these calli and transferred to
rooting medium. Rooted plantlets grew vigorously in vitro and flowering was frequently observed. However, establishment of
rooted shoots in soil was unsuccessful. Hybrid identity was confirmed by intermediate shoot morphology, RAPD marker analysis,
and flow cytometric estimation of nuclear DNA content.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Alternaria black spot Brassica oleracea Camelina sativa disease resistance false flax protoplast fusion |
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