成年兔睾丸支持细胞的分离纯化研究

本试验以5月龄公兔为试验动物,去除睾丸被膜及血管,分离出完整的曲精细管后 用0．5％胰蛋白酶和0．1％胶原酶消化并进行低渗处理,将制成的细胞悬液植入25 cm2的培 养瓶,于38．7℃、5％CO2条件下培养,结果表明:经1:3Hanks超纯水低渗处理后,离体支持 细胞纯度和活率分别达到72．04％和84．5％,显著提高了离体支持细胞的获取量和活率。

Isolation and Purification of Testis Sertoli Cells in Adult Rabbit

Decapsulated and removed tests from 5-mo-old rabbit, then the whole seminiferous tubules were dispersed. The subsequent enzymatic digestion was used with 0. 5% trypsin and 0. 1% collagenase, the resulting tissues were made into cell suspension and subjected to hypotonic shock, after that, the Sertoli cells were cultured at 38. 7 C with 5% CO2 in 25 cm2 flasks. The result showed that, after treated with hypotonic shock in 1/3 group, the purity and viability of isolated Sertoli cells reached to 72. 04% and 84. 5% respectively. It increased the quality and viability of isolated Sertoli cells greatly.