| 番茄cry1基因果实特异性植物表达-载体的构建及其转化番茄的研究 |
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| 引用本文： | 孙艳.番茄cry1基因果实特异性植物表达-载体的构建及其转化番茄的研究[J].安徽农业科学,2007,35(3):665-666,668. |
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| 作者姓名： | 孙艳 |
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| 作者单位： | 四川大学生命科学学院生物资源与生态环境教育部重点实验室,四川成都,610064 |
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| 摘 要： | 隐花色素(cryptochrome)是一类对UV-A/蓝光作出应答的光受体.果实特异性植物表达载体是用在番茄果实中特异表达的Lefsm1基因的启动子(fsp)替换质粒pBI121原有的35S启动子构建而成(称为pBI121 fsp).将番茄隐花色素家族成员之一cryptochrome1中465 bp特异片段导入到植物栽体pBI121 fsp构建成果实特异性表达的RNAi植物表达载体(pBlI21fsp-cryI),通过根癌农杆菌介导转入番茄子叶,经组织培养成功获得转基因植株.为后续对CRY1与番茄果实中抗氧化剂番茄红素之间的关系研究提供了材料.
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| 关 键 词： | 隐花色素 果实特异性启动子 子叶 番茄转化 |
| 文章编号： | 0517-6611（2007）03-00665-02 |
| 修稿时间： | 2006-10-31 |
Construction of Plant Expression Vector with Specific Fruit Promoter of Gene Cry1 in Transgenic Tomato |
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| SUN Yan.Construction of Plant Expression Vector with Specific Fruit Promoter of Gene Cry1 in Transgenic Tomato[J].Journal of Anhui Agricultural Sciences,2007,35(3):665-666,668. |
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| Authors: | SUN Yan |
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| Institution: | Key Laboratory of Bio-resources and Eco-environment of Ministry of Agriculture, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064 |
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| Abstract: | Cryptochrome is one of the UV-A/blue light photoreceptors.A part of gene cry1 was inserted into the T-DNA of the binary plant expression vector with pBI121 plasmid,which contained specific fruit promoter.The cotyledon of tomato was transformed with agrobacterium tumefaciens EHA105,some regenerated tomato plants were obtained through the tissue culture. |
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| Keywords: | Cyptochrome Specific fruit promoter Cotyledon Tomato transformation |
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