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大白菜雄性核不育复等位基因的鉴定
引用本文:杨皓宁,冯辉,李承彧,王玉刚.大白菜雄性核不育复等位基因的鉴定[J].沈阳农业大学学报,2008,39(6).
作者姓名:杨皓宁  冯辉  李承彧  王玉刚
作者单位:沈阳农业大学园艺学院,沈阳,110161
基金项目:国家自然科学基金项目 , 辽宁省博士启动基金资助项目  
摘    要:为了摸清大白菜核不育复等位基因在3种生态型大白菜品系中的分布特性.利用基因型为MsMs的大白菜纯合显性雄性不育株为母本.与68个正常可育的直筒、卵圆和平头型大白菜可育品系杂交.统计后代育性分离比例,鉴定各品系基因型,计算不同生态型可育品系在核不育位点上的基因频率和基因型频率.结果表明:直筒型大白菜可育品系以纯合隐性基因型msms为主,占71.43%:可育基因ms频率为76.79%,恢复基Ms频率为23.21%.卵圆型大白菜可育品系3种基因型Ms/MsfMsfms和msms分别占42.42%.39.40%和18.18%;Msf基冈频率为62.12%,ms基因频率为37.88%.平头型大白菜可育品系以纯合隐性基因型msms为主,占57.14%:Msf基因频率为33.33%,ms基因频率为66.67%.直简型和平头型大白菜可育品系的核不育复等位基因位点上以ms基因为主.而卵圆型大白菜可育品系的核不育复等位基因位点上以Msf基因为主.

关 键 词:大白菜  核基因雄性不育  复等位基因  基因频率  基因型频率

Identification of Multiple Male Sterile Allele in Chinese Cabbage
YANG Hao-ning,FENG Hui,LI Cheng-yu,WANG Yu-gang.Identification of Multiple Male Sterile Allele in Chinese Cabbage[J].Journal of Shenyang Agricultural University,2008,39(6).
Authors:YANG Hao-ning  FENG Hui  LI Cheng-yu  WANG Yu-gang
Institution:YANG Hao-ning,FENG Hui,LI Cheng-yu,WANG Yu-gang(College of Horticulture,Shenyang Agricultural University,Shenyang 110161,China)
Abstract:The objective of this experiment was to investigate the characteristic distribution of nuclear sterile multiple allele genes of Chinese cabbage in 3 ecotype cabbage lines.A dominant male sterile Chinese cabbage MsMs was applied as test cross parent to make hybridizations with 68 male fertile cabbage lines belonging to Straight-tubbish,Ovate,or Level-head ecotypes.Through calculating the fertility segregation ratio of the progenies,and identifying the genotype of each male fertile lines,the gene and genotype...
Keywords:Chinese cabbage  genetic male sterility  multiple allele  gene frequency  genotype frequency  
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