| 牛初乳中乳铁蛋白粗提工艺的优化研究 |
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| 引用本文： | 罗芳,刘成国,徐钦,成亮.牛初乳中乳铁蛋白粗提工艺的优化研究[J].乳业科学与技术,2010,33(1):11-14. |
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| 作者姓名： | 罗芳 刘成国 徐钦 成亮 |
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| 作者单位： | 湖南农业大学食品科学技术学院,湖南长沙,410128 |
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| 摘 要： | 利用盐析和有机溶剂沉淀相结合的方法对牛初乳中的乳铁蛋白进行初步的分离,采用单因素试验和正交试验的方法对影响分离效果的因素进行了试验研究,研究结果表明:用pH值8.5、80%的硫酸铵溶液进行盐析,然后用60%的乙醇浓度进行沉淀,分离的效果较好。SDS-PAGE凝胶电泳结果表明,乳铁蛋白的纯度大约为48%,分子量大约为78000Da。
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| 关 键 词： | 乳铁蛋白 牛初乳 盐析 分离 |
Study on the Optimization of Technique of Crude Extracting Lactoferrin from Bovine Colostrum |
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| Luo Fang,Liu Chengguo,Xu Qin,Cheng Liang.Study on the Optimization of Technique of Crude Extracting Lactoferrin from Bovine Colostrum[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2010,33(1):11-14. |
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| Authors: | Luo Fang Liu Chengguo Xu Qin Cheng Liang |
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| Institution: | Luo Fang,Liu Chengguo,Xu Qin,Cheng Liang College of Food Science , Technology of Hunan Agricultural University,Changsha of Hunan 410128,China |
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| Abstract: | Lactoferrin was crude extracted by salt-out and organic reagent precipitation from bovine colostrums,and the optimal isolating conditions were obtained through the single factor and orthogonal experiments.The result showed that salt-out at pH 8.5 with 80 %(NH4)2SO4,precipitation with 60 % ethanol.The result from SDS-PAGE showed that the purity of the lactoferrin was 48 %,and the molecular weight was about 78 000 Da. |
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| Keywords: | Lactoferrin bovine colostrum salt-out separation |
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