| 产氢菌AUH-BG208的分离鉴定与产氢特性 |
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| 引用本文： | 樊进茹,王世英,李佳,成志远,倪鑫,王秀伶. 产氢菌AUH-BG208的分离鉴定与产氢特性[J]. 河北农业大学学报, 2010, 33(2) |
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| 作者姓名： | 樊进茹 王世英 李佳 成志远 倪鑫 王秀伶 |
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| 作者单位： | 河北农业大学,生命科学学院,河北,保定,071001;河北农业大学,生命科学学院,河北,保定,071001;河北农业大学,生命科学学院,河北,保定,071001;河北农业大学,生命科学学院,河北,保定,071001;河北农业大学,生命科学学院,河北,保定,071001;河北农业大学,生命科学学院,河北,保定,071001 |
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| 基金项目： | 国家自然科学基金资助项目(30570035) |
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| 摘 要： | 从白冠长尾雉粪样中分离出一株产氢量高的细菌菌株,命名为AUH-BG208。通过革兰氏染色和耐氧性测定,菌株AUH-BG208为革兰氏阴性兼性厌氧菌株。菌株AUH-BG208形态学及生理生化特征与克雷伯氏属一致,通过菌株16S rDNA序列分析,发现该菌株与肺炎克雷伯氏菌Klebsiella pneumoniae(AJ233420)相似性为100%。此外,对菌株AUH-BG208在不同条件下的产氢特性进行了测定。结果表明:该菌株在起始pH值为7.0,葡萄糖浓度为40 g/L,以酵母粉和硝酸钾为氮源时产氢能力最强,产氢量为1 900 mL/L。
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| 关 键 词： | 产氢细菌 分离 鉴定 产氢特性 |
Isolation and identification of hydrogen-producing bacterium AUH-BG 208 and hydrogen-producing capacity under different conditions |
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| FAN Jin-ru,WANG Shi-ying,LI Jia,CHENG Zhi-yuan,NI Xin,WANG Xiu-ling. Isolation and identification of hydrogen-producing bacterium AUH-BG 208 and hydrogen-producing capacity under different conditions[J]. Journal of Agricultural University of Hebei, 2010, 33(2) |
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| Authors: | FAN Jin-ru WANG Shi-ying LI Jia CHENG Zhi-yuan NI Xin WANG Xiu-ling |
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| Affiliation: | College of Life Sciences;Agricultural University of Hebei;Baoding 071001;China |
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| Abstract: | One bacterium designated AUH-BG208 with higher hydrogen-producing capacity was isolated from Syrmaticus reevesii feces.Bacterial strain AUH-BG208 is a facultative gram-negative bacterium based on gram-staining and oxygen-endurance ability test.Physiological and biochemical characteristics and morphological study showed that bacterial strain AUH-BG208 had the same properties of that of genus Klebsiella.BLAST search on the GenBank revealed that the full-length of 16S rDNA gene sequence of strain AUH-BG208 has... |
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| Keywords: | hydrogen-producing bacteria isolation identification hydrogen-producing property |
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