| RP-HPLC法测定健肝乐颗粒中芍药苷的含量 |
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| 引用本文： | 张雪荣,张静,段立华.RP-HPLC法测定健肝乐颗粒中芍药苷的含量[J].安徽农业科学,2009,37(21):9834-9835. |
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| 作者姓名： | 张雪荣 张静 段立华 |
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| 作者单位： | 河北化工医药职业技术学院,河北石家庄,050026;河北化工医药职业技术学院,河北石家庄,050026;河北化工医药职业技术学院,河北石家庄,050026 |
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| 摘 要： | 目的]建立健肝乐颗粒中芍药苷的含量测定方法。方法]采用RP—HPLC法，色谱柱为ODS2-C18柱（250mm×4．6mm，5μm），流动相为乙腈-水（15：85），流速为1．0ml／min，检测渡长为230mm。结果]芍药苷在0．16-0．83．峙范围内与峰面积呈良好线性关系。加样回收率为99．20％，RSD为1．2％（n=6）。结论]采用高效液相法对健肝乐颗粒中芍药苷进行含量测定，具有操作简单、准确、重复性好、精密度高、专属性强的特点，可用于健肝乐颗粒的质量检测。
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| 关 键 词： | RP-HPLC法 健肝乐颗粒 芍药苷 含量测定 |
Determination of Paeoniflor in Jianganle Particle by RP-HPLC |
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| ZHANG Xue-rong et al.Determination of Paeoniflor in Jianganle Particle by RP-HPLC[J].Journal of Anhui Agricultural Sciences,2009,37(21):9834-9835. |
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| Authors: | ZHANG Xue-rong |
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| Institution: | ZHANG Xue-rong et al (Hebei Chemical , Pharmaceutical College,Shijiazhuang,Hebei 050026) |
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| Abstract: | Objective]The research aimed to establish a method for the determination of paeoniflor in Jianganle particle by RP-HPLC. Method]The content of paeoniflor in Jianganle particle was determinated by using RP-HPLC. The separation was performed on a ODS2- C18 column(250 mm×4.6 mm, 5 μm). The mobile phase was cetonitrile-water (15∶85)at the flow rate of 1.0 ml/min. The defection wavelength was 230 nm. Result]The linear range of paeoniflorin was in the range of 0.16-0.83 μg;The addition recovery rate was 99.20%, RSD=1.2%(n=6). Conclusion]The method for the determination of paeoniflor in Jianganle particle by RP-HPLC is simple, rapid, exclusive and accurate, and it is suitable for determination of paeoniflor of Jianganle particle. |
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| Keywords: | RP-HPLC Jianganle particle Paeoniflor Content determination |
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