| 总DNA导入水稻后变异系及供体特异带DNA片段的核苷酸序列比较 |
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| 引用本文： | 赵炳然,贾建航,王倩,王斌,袁隆平. 总DNA导入水稻后变异系及供体特异带DNA片段的核苷酸序列比较[J]. 杂交水稻, 2001, 16(2): 46-49 |
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| 作者姓名： | 赵炳然 贾建航 王倩 王斌 袁隆平 |
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| 作者单位： | 1. 国家杂交水稻工程技术研究中心,
2. 中国科学院遗传研究所, |
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| 基金项目： | 湖南省科委重点科技攻关项目 |
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| 摘 要： | 将小粒野生稻（Oryza minuta)总DNA导入杂交水稻保持系V20B获得变异系，经RAPD分析找到了受体中不存在而变异系及供体中存在的特异常。将自变异系与供体扩增出的长均为975bp的特异DNA片段分别回收、克隆、测序，发现两者核苷酸序列极为相似，从而进一步证明了远缘资源特异DNA片段向水稻的转移；同时发现两者间存在29个碱在差异（包括转换、颠换、插入及缺失4种类型），表明供体特异DNA片段的导入不仅可能使供体特异性状在变异系中表达，也可能由于个别碱基的突变而使受体产生新性状。
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| 关 键 词： | 水稻 野生稻 DNA转移 特异DNA片段 核苷酸序列 变异系 供体 |
| 文章编号： | 1005-3956(2001)02-0046-04 |
| 修稿时间： | 2000-10-18 |
Comparison of theNucleotide Sequence of the Specific DNA Fragments between the Variant Line and Its Donor |
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| ZHAO Bing-ran,JIA Jian-hang,WANG Qian,WANG Bin,YUAN Long-ping. Comparison of theNucleotide Sequence of the Specific DNA Fragments between the Variant Line and Its Donor[J]. Hybrid Rice, 2001, 16(2): 46-49 |
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| Authors: | ZHAO Bing-ran JIA Jian-hang WANG Qian WANG Bin YUAN Long-ping |
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| Affiliation: | ZHAO Bing-ran1,JIA Jian-hang2,WANG Qian2,WANG Bin2,YUAN Long-ping1 |
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| Abstract: | Total DNA of Oryza minuta was injected into the spike-stalks of V20B (a maintainer line of hybrid rice ) during a suitable period. Variants were obtained in the first generation. Two specific DNA fragments (not found in V20B) of 97C-77(a strain of the 7th generation from a variant) and O.minuta from RAPD analysis were sequenced. Sequences of the two DNA fragments were identical except for the differences at 29 base positions. The results proved further that specific DNA fragment of O.minuta had been transferred into V20B, and indicated that the specific exogenous DNA fragments may even cause the recipient obtaining new variant characters for changes of their base sequences. |
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| Keywords: | rice total DNA of wild rice DNA transfer specific DNA fragment nucleotide sequence |
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