Pollen morphology as fertility predictor in hybrid tea roses |
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Authors: | Luca Pipino Marie-Christine Van Labeke Andrea Mansuino Valentina Scariot Annalisa Giovannini Leen Leus |
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Institution: | (1) Department of Agronomy, Forest and Land Management, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco, TO, Italy;(2) Department of Plant Production, Ghent University, Coupure Links 653, 9000 Ghent, Belgium;(3) NIRP International, Az. Agricola di Ghione L. & Figli, via San Rocco 1, Fraz. Bevera, 18039 Ventimiglia, IM, Italy;(4) CRA-FSO, Research Unit for Floriculture and Ornamental Species, Corso Inglesi 508, 18038 Sanremo, IM, Italy;(5) Plant Sciences Unit, ILVO, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090 Melle, Belgium; |
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Abstract: | Fertility of hybrid tea roses is often reduced due to their interspecific origin but also to intensive inbreeding. New genotypes
used as pollen donors represent an economic risk for a breeding programme, as their influence on seed production is unknown.
In this study 11 cut rose genotypes were selected from a company database as high fertile or low fertile male parents, according
to the number of seeds per hybridisation. Pollen morphology and in vitro germination of the selected genotypes were characterised.
Pollen was either small (mean diameter <30 μm), shrunken, and irregular (abnormal), or large (mean diameter >30 μm), elliptical
and crossed by furrows (normal). High correlations were found between the number of seeds produced per hybridisation and the
pollen diameter (r = 0.94) or the percentage of normal pollen (r = 0.96). In order to evaluate the predictive power of the models, we conducted regression analyses and performed a validation
experiment on genotypes not present in the database and without background information on fertility. Pollen diameter and percentage
of normal pollen were characterised and fitted in the regression models for seed set predictions. Validation with an independent
dataset gave a good prediction for 83.3% of the data. This indicates that using either the mean pollen diameter or the percentage
of normal pollen resulted in effective fertility prediction. This tool could enhance the genetic variability in crossings
between hybrid tea roses, thus creating possibilities for less economically risky exploitation of new tetraploid genotypes
as male parents. |
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