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| 草莓果实磷脂酶D活性的测定 | |
| 引用本文： | 袁海英,陈力耕,李疆. 草莓果实磷脂酶D活性的测定[J]. 果树学报, 2005, 22(5): 579-581 |
| 作者姓名： | 袁海英 陈力耕 李疆 |
| 作者单位： | 1. 浙江大学园艺系·农业部园艺植物生长发育与生物技术重点开放实验室,杭州,310029;新疆农业大学园艺系,乌鲁木齐,830052 2. 浙江大学园艺系·农业部园艺植物生长发育与生物技术重点开放实验室,杭州,310029 3. 新疆农业大学园艺系,乌鲁木齐,830052 4. 圭尔夫大学植物农学系,加拿大 圭尔夫 N1G 2W1 |
| 基金项目： | 国家留学基金及加拿大自然科学与工程协会基金。 |
| 摘 要： | 以草莓果实为试材,建立了适合草莓果实组织磷脂酶D制备及活性测定的技术体系。以放射标记的磷脂酰胆碱为底物,在1mL反应体系中,膜蛋白的含量以5-10μg为宜;25℃反应温度下,反应时间以5-10min为宜;反应体系的适宜pH为6.5-7。对草莓果实不同组分磷脂酶D酶学特性的进一步分析认为,该技术体系的建立有助于进一步探讨磷脂酶D在草莓果实发育成熟和衰老进程中的作用。 |
| 关 键 词： | 草莓 磷脂酶D 测定方法 |
| 文章编号： | 1009-9980(2005)05-579-03 |
| 修稿时间： | 2004-12-20 |
Improved method for detecting phospholipase D activity from strawberry (Fragaria ananassa) Fruit |
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| YUAN Hai-ying,CHEN Li-geng,Li Jiang,Gopinadhan Paliyath. Improved method for detecting phospholipase D activity from strawberry (Fragaria ananassa) Fruit[J]. Journal of Fruit Science, 2005, 22(5): 579-581 | |
| Authors: | YUAN Hai-ying CHEN Li-geng Li Jiang Gopinadhan Paliyath |
| Affiliation: | YUAN Hai-ying1,2,CHEN Li-geng1,Li Jiang2,Gopinadhan Paliyath3 |
| Abstract: | Strawberry fruit were used to establish the improved method for detecting PLD activity. Using radio-labeled phosphatidylcholine as substrate, the proper protein content in 1 mL reaction system is 5-10 μg; the reaction time is better in 5-10 min at 25℃ and the suitable pH for strawberry fruits is 6.5-7. Further analysis on strawberry fruit PLD from different fractions suggests that this method will help to elucidate the potential roles of PLD in strawberry fruit ripening and senescence process. |
| Keywords: | Strawberry Phospholipase D Detection method |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |