The effect of tannin treatment and subsequent urea supplementation of sunflower meal on the in vitro digestibility of its crude protein for ruminants

The effect of tannin treatment (TSFM) and subsequent urea supplementation (TSFM + U) on the digestibility of sunflower meal (SFM) protein was studied by means of a three-phase laboratory method simulating the ruminant's digestion. Under the influence of the ruminal fluid 67% of SFM protein, 62% of TSFM protein and 58% of TSFM + U protein was degraded. The pepsin and pancreatin digestibilities of rumen undegraded protein (UDP) were as follow: 58.2% (SFM), 56.6% (TSFM), 43.3% (TSFM + U), and 22.0% (SFM), 28.8% (TSFM), 17.5% (TSFM + U), respectively. The four fractions (dissolved protein, oligopeptides, amino acids, and ammonia) of rumen degradable protein (DP) were also determined: 85 to 92% of rumen degradable protein was recovered in these four fractions. Owing to tannic acid treatment the quantity of dissolved protein decreased and that of oligopeptides increased.