| 14株网状内皮组织增生症病毒中国分离株gp90基因的分子特征 |
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| 引用本文： | 邓小芸,祁小乐,高玉龙,秦立廷,高立,吴关,王永强,高宏雷,王笑梅. 14株网状内皮组织增生症病毒中国分离株gp90基因的分子特征[J]. 农业科学与技术, 2011, 0(12): 1954-1957 |
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| 作者姓名： | 邓小芸 祁小乐 高玉龙 秦立廷 高立 吴关 王永强 高宏雷 王笑梅 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽传染病研究室;黑龙江科技职业学院 |
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| 基金项目： | Supported by Earmarked Fund for Modern Agro-industry Technology Research System of China ( nycytx-42-G3-01)~~ |
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| 摘 要： | [目的]研究十四株网状内皮组织增生症病毒中国分离株gp90基因的分子特征。[方法]对分离于我国不同商品蛋鸡场的14株REV的表面囊膜基因gp90进行了扩增和序列测定。[结果]序列分析表明,这14株REV不同于我国早期分离毒株与亚型3毒株亲缘关系较近。另外发现,这14株REV与美国和我国台湾的流行毒株同源性较高。[结论]本研究对深入研究我国REV的流行和遗传演化意义重大。
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| 关 键 词： | 网状内皮组织增生症病毒（REV） gp90 序列分析 |
Molecular Characteristics of gp90 Gene of 14 Reticuloendotheliosis Viruses Isolated in China |
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| DENG Xiao-yun,QI Xiao-le,GAO Yu-long,QIN Li-ting,GAO Li,WU Guan,WANG Yong-qiang,GAO Hong-lei,WANG Xiao-mei. Molecular Characteristics of gp90 Gene of 14 Reticuloendotheliosis Viruses Isolated in China[J]. Agricultural Science ＆ Technology, 2011, 0(12): 1954-1957 |
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| Authors: | DENG Xiao-yun QI Xiao-le GAO Yu-long QIN Li-ting GAO Li WU Guan WANG Yong-qiang GAO Hong-lei WANG Xiao-mei |
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| Affiliation: | 1.Division of Avian Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,the Chinese Academy of Agricultural Sciences,Harbin 150001;2.Heilongjiang Vocational College of Science and Technology,Shuangcheng 150111 |
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| Abstract: | [Objective] The paper was to study molecular characteristics of gp90 gene of 14 Reticuloendotheliosis viruses isolated in China.[Method] The surface envelop gene gp90 of 14 REV strains isolated from different commercial layer farms in China were amplified,and their nucleotide sequences were determined.[Result] Sequence analysis showes that 14 REV strains are more identical to the subtype 3 isolates than to the early Chinese REV isolates.In addition,14 REV strains have a high identity with some REV strains in US and Taiwan.[Conclusion] The study provided necessary information for further understanding the evolution of REV. |
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| Keywords: | Reticuloendotheliosis virus(REV) gp90 Sequence analysis |
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