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Analysis of gene sequences for the nucleocapsid protein from Tomato spotted wilt virus for promoting RNA-mediated cross-protection using the Potato virus X vector system
Authors:Shoji?Sonoda  author-information"  >  author-information__contact u-icon-before"  >  mailto:sonodas@rib.okayama-u.ac.jp"   title="  sonodas@rib.okayama-u.ac.jp"   itemprop="  email"   data-track="  click"   data-track-action="  Email author"   data-track-label="  "  >Email author,Hisaaki?Tsumuki
Affiliation:(1) Research Institute for Bioresources, Okayama University, Kurashiki, Okayama, 710-0046, Japan
Abstract:Virus interactions between Tomato spotted wilt virus (TSWV) and Potato virus X (PVX) containing the nucleocapsid protein (N) gene sequences were examined to evaluate the capacity of the N gene sequences from TSWV to promote RNA-mediated cross-protection. Plants simultaneously inoculated with TSWV and PVX containing the 3prime 96thinspbp of the N gene were highly resistant to TSWV infection, whereas no such resistance was observed in plants inoculated with TSWV and PVX containing the 5prime 96thinspbp. These results suggest that the 3prime portion of the N gene has a higher capacity for promoting RNA-mediated cross-protection of TSWV.
Keywords:Cross-protection   Nicotiana benthamiana   RNA silencing   Tospovirus   Virus resistance
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