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| 口蹄疫病毒P12X3C3D多基因编码蛋白在昆虫细胞中的表达与检测 | |
| 引用本文： | 冷青文,郭慧琛,刘在新,谢庆阁,张居农.口蹄疫病毒P12X3C3D多基因编码蛋白在昆虫细胞中的表达与检测[J].中国兽医学报,2006,26(3):229-231. |
| 作者姓名： | 冷青文 郭慧琛 刘在新 谢庆阁 张居农 |
| 作者单位： | 1. 中国农业科学院,兰州兽医研究所,农业部病毒学重点实验室,甘肃,兰州,730046;石河子大学,动物科技学院,新疆,石河子,832003 2. 中国农业科学院,兰州兽医研究所,农业部病毒学重点实验室,甘肃,兰州,730046 3. 石河子大学,动物科技学院,新疆,石河子,832003 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 利用杆状病毒表达系统构建了包含有口蹄疫病毒（FMDV）P12X3C3D多基因片段的重组杆状病毒。将该病毒感染Sf9细胞后，利用SDS—PAGE及夹心ELISA方法检测目的蛋白的表达。结果表明，重组杆状病毒能够表达FMDV目的蛋白，该表达产物能被FMDV阳性血清识别，具有一定的反应原性。 |
| 关 键 词： | 口蹄疫病毒 P12X3C3D多基因 杆状病毒表达系统 真核表达 |
| 文章编号： | 1005-4545(2006)03-0229-03 |
| 收稿时间： | 2004-09-20 |
| 修稿时间： | 2004年9月20日 |
Expression and Detection of the Multi-gene P12X3C3D of Foot-and-Mouth Disease Virus in Insect Cells |
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| LENG Qing-wen,GUO Hui-chen,LIU Zai-xin,XIE Qing-ge,ZHANG Ju-nong.Expression and Detection of the Multi-gene P12X3C3D of Foot-and-Mouth Disease Virus in Insect Cells[J].Chinese Journal of Veterinary Science,2006,26(3):229-231. | |
| Authors: | LENG Qing-wen GUO Hui-chen LIU Zai-xin XIE Qing-ge ZHANG Ju-nong |
| Institution: | 1. Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China ; 2. College of Animal Science and Technology,Shihezi University, Shihezi 832003, China |
| Abstract: | By means of techniques of Bac-to-Bac baculovirus expression system, the recombinant baculovirus particles containing the muhi-gene P12X3C3D of FMDV, were obtained. Fresh insect Sf9 cells were infected with the recombinant baculovirus to express the proteins of FMDV. The results showed that the muhi-gene was successfully expressed in insect Sf9 cells and its products can be recognized by the positive bovine serum of FMDV. The amount of target proteins accounts for 30%-40% of the total protein in cells lysate. The research provided an available material for development of the gene engineering vaccine against FMD. |
| Keywords: | FMDV muhi-gene P12X3C3D bac-to-bac baculovirus expression system eukaryotic expression |
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