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| 复方黄连乳腺炎注射液及小檗碱抑菌试验 | |
| 引用本文： | 白丽欣,郝满良,叶宝娜,秦建华,张振红,谢菲.复方黄连乳腺炎注射液及小檗碱抑菌试验[J].河北农业大学学报,2010,33(2). |
| 作者姓名： | 白丽欣 郝满良 叶宝娜 秦建华 张振红 谢菲 |
| 作者单位： | 1. 河北农业大学,动物科技学院,河北,保定,071001 2. 河北青县畜牧局,河北,青县,062650 3. 山东荣成出入境检验检疫局,山东,荣成,264300 |
| 基金项目： | 国家十一五重大专项子专题“华北农区奶牛疾病综合防治技术应用与示范”(2006BAD04A10-4);;河北省重大技术创新专项计划河北省“千万吨奶工程”支撑技术研究(课题编号:07227146Z-4);;河北省教育厅2009科研计划项目(2009453) |
| 摘 要： | 为测试复方黄连乳腺炎注射液以其主要有效成分小檗碱体内和体外抑菌效果。采用琼脂平板扩散法(打孔法)和试管二倍稀释法,用复方黄连乳腺炎注射液对引起奶牛乳房炎的3种主要致病菌(金黄色葡萄球菌、大肠杆菌、无乳链球菌)进行体外抑菌试验,结果显示对金黄色葡萄球菌抑菌圈直径为42.3 mm,对大肠杆菌抑菌圈直径为35.2 mm,对无乳链球菌抑菌圈直径为41.5 mm。采用小鼠乳腺炎模型对小檗碱体内抑菌效果和对小鼠乳腺组织中细胞因子的影响进行试验,结果显示:与模型组相比,低剂量组、高剂量组在接菌12~48 h后小鼠乳腺组织中的细菌数均极显著低于模型组(P0.01);低剂量组、高剂量组小鼠乳腺组织TNF-α在接菌24 h后极显著低于模型组(P0.01);接菌24~36 h后,低剂量组和高剂量组乳腺组织IFN-γ含量极显著低于模型组(P0.01)。结果表明:复方黄连乳腺炎注射液对金黄色葡萄球菌、大肠杆菌、无乳链球菌均有较好抑菌效果,其主要有效成分小檗碱能明显抑制小鼠乳腺内金黄色葡萄球菌的增殖,降低乳腺组织TNF-α、IFN-γ水平,从而减轻炎症介质对乳腺的病理损伤。 |
| 关 键 词： | 复方黄连乳腺炎注射液 小檗碱 抑菌试验 TNF-α IFN-γ |
Bacteriostasis test of berberine and compound Rhizoma coptidis injection on mastitis |
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| BAI Li-xin,HAO Man-liang,YE Bao-na,QIN Jian-hua,ZHANG Zhen-hong,XIE Fei.Bacteriostasis test of berberine and compound Rhizoma coptidis injection on mastitis[J].Journal of Agricultural University of Hebei,2010,33(2). | |
| Authors: | BAI Li-xin HAO Man-liang YE Bao-na QIN Jian-hua ZHANG Zhen-hong XIE Fei |
| Institution: | 1.College of Animal Science and Technology;Agricultural University of Hebei;Baoding 071001;China;2.Bureau of Animal Husbandry of Qingxian;Qingxian 062650;3.Rongcheng Entry-exit Inspection and Quarantine Bureau;Rongcheng 264300;China |
| Abstract: | This study aims to test the bacteriostasis effect of compound Rhizoma coptidis injection on mastitis and Berberine of main active ingredient.By agar diffusion method(punch method) and two-fold dilution method in test tube,in vitro bacteriostasis test about Staphylococcus aureus,Escherichia coli,Streptococcus agalactiae of compound Rhizoma coptidis injection on mastitis were investigated.Results showed that the bacteriostasis diameters of compound Rhizoma coptidis injection on Staphylococcus aureus,E.coli,S.... |
| Keywords: | compound Rhizoma coptidis injection berberine bacteriostasis test |
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