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| 新疆核桃品种试管苗不定根发生的影响因素研究 | |
| 引用本文： | 胡石开,王晓军,郝秀英,唐晓义,李静,牛力涛,努尔·波拉提.新疆核桃品种试管苗不定根发生的影响因素研究[J].北方园艺,2009(8). |
| 作者姓名： | 胡石开 王晓军 郝秀英 唐晓义 李静 牛力涛 努尔·波拉提 |
| 作者单位： | 1. 中国科学院,新疆理化技术研究所,新疆,乌鲁木齐,830011;中国科学院,研究生院,北京,100039 2. 中国科学院,新疆理化技术研究所,新疆,乌鲁木齐,830011 3. 新疆农业科学院,微生物研究所,新疆,乌鲁木齐,830091 |
| 基金项目： | 中国科学院西部行动高新技术资助项目(KGCX2-SW-506) |
| 摘 要： | 以新疆2个优良核桃品种新温185号、新温2号为试材,对影响试管苗的不定根发生的影响因素进行了研究。结果表明:基本培养基、外源IBA水平、光周期及黑暗条件对不定根发生具有一定的影响。选取生长旺盛的试管苗,采用一步浸蘸诱导生根法,即将试管苗放入经常规灭菌处理过的80 mg/L的IBA溶液中浸蘸处理75 min,然后转入不含任何激素的1/4 DKW中,黑暗处理12 d后置于光照下生根。利用这一方法快速诱导了试管苗生根,获得了高达90.90%的生根率,生根指数达254.44。 |
| 关 键 词： | 核桃品种 生根 离体培养 新疆 |
Factors Affecting in vitro Rooting of Xinjiang Walnut Cultivars |
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| HU Shi-kai,WANG Xiao-jun,HAO Xiu-ying,TANG Xiao-yi,Li Jing,NIU Li-tao,NUR·Bolat.Factors Affecting in vitro Rooting of Xinjiang Walnut Cultivars[J].Northern Horticulture,2009(8). | |
| Authors: | HU Shi-kai WANG Xiao-jun HAO Xiu-ying TANG Xiao-yi Li Jing NIU Li-tao NUR·Bolat |
| Institution: | 1.Xinjiang Technical Institute of Physics and Chemistry;Chinese Academy of Sciences;Urumqi;Xinjiang 830011;China;2.Graduate University of Chinese Academy of Sciences;Beijing 100039;3.Institute of Microbiology;Xinjiang Academy of Agricultural Science;Xinjiang 830091;China |
| Abstract: | The factors affecting in vitro rooting of Xinjiang walnut cultivars-Xinwen 185 and Xinwen 2 were researched.The results showed that the rooting was related to the basic medium,the level of foreign IBA,light and dark conditions.The rooting rate of 90.90%and root index of 254.44 were achieved in two cultivars by using a step-induced root dip method that was firstly induced in 80 mg/L of IBA solution for 75 minutes,and then transferred to the 1/4DKW medium which did not contain any hormone,and then took to ind... |
| Keywords: | Juglans regia L cultivars Rooting In vitro culture Xinjiang |
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