| 耐受甲醛并能利用甲醇细菌的分离及其基因组文库的构建 |
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| 引用本文： | 陈丽梅,殷飞,李昆志,魏云林,林连兵. 耐受甲醛并能利用甲醇细菌的分离及其基因组文库的构建[J]. 安徽农业科学, 2008, 36(3): 978-981,1009 |
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| 作者姓名： | 陈丽梅 殷飞 李昆志 魏云林 林连兵 |
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| 作者单位： | 昆明理工大学生物工程技术研究中心,云南昆明,650224;昆明理工大学生物工程技术研究中心,云南昆明,650224;昆明理工大学生物工程技术研究中心,云南昆明,650224;昆明理工大学生物工程技术研究中心,云南昆明,650224;昆明理工大学生物工程技术研究中心,云南昆明,650224 |
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| 基金项目： | 国家自然科学基金项目（30670163）;教育部留学回国人员科研启动基金项目（2005-55）;云南省中青年学术与技术带头人培养费（2004PY01-5）和昆明理工大学人才培养费联合资助.鸣谢 昆明理工大学生物工程技术研究中心的陆小波、陈责元、胡清泉、季秀玲、井升荣、李光等的帮助与指导. |
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| 摘 要： | 从污水样品筛选出能利用甲醇的菌株A和B,经16S rDNA测序分析,鉴定为芽孢杆菌(Bacillussp.KM01)和肺炎克氏杆菌(Kleb-siella pneumoniae)。甲醛耐受能力的测试表明,这两种分离菌对甲醛具有较强的耐受能力,能在含8~15 mmol/L甲醛的LB培养基上生长。Southern杂交分析结果说明,这两株菌的基因组中有兼性甲基营养菌6-磷酸己酮糖合成酶(HPS)和6-磷酸果糖异构酶(PHI)基因的同源序列。以pUC118为载体构建了这两种分离菌的基因组文库,进一步的检测结果说明所构建的基因组文库质量符合要求。
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| 关 键 词： | 甲醇 甲基营养菌 甲醛耐受性 16S rDNA 基因组文库 |
| 文章编号： | 0517-6611（2008）03-00978-04 |
| 收稿时间： | 2007-09-25 |
| 修稿时间： | 2007-09-25 |
Research on the Isolation of the HCHO-resistance Bacteria with CH3OH-utilization Ability and the Construction of Genomic Library |
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| CHEN Li-mei et al. Research on the Isolation of the HCHO-resistance Bacteria with CH3OH-utilization Ability and the Construction of Genomic Library[J]. Journal of Anhui Agricultural Sciences, 2008, 36(3): 978-981,1009 |
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| Authors: | CHEN Li-mei et al |
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| Abstract: | The strain A and B with CH3OH-utilization ability were isolated from sewage samples.By sequence analyses for 16S rDNA,the two isolated strains were identified as Bacillus sp.KM01 and Klebsiella pneumoniae,respectively.The test with HCHO showed they were well tolerant to HCHO and could grow on LB medium with 8～15 mmol/L HCHO.Southern hybridization analyses revealed that the homologs of 3-Hexulose-6-phosphate synthase(HPS) and 6-phospho-3-hexuloisomerase (PHI) genes from a facultative methylotrophic bacterium,Mycobacterium gastri MB19,were presented in the genomes of the isolated strains.pUC118 being used as the vector,genomic DNA libraries were constructed for the isolated strains.The further analyses showed that the quality of the libraries was satisfied for the requirements for gene screening. |
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| Keywords: | CH3OH Methylotroph HCHO-resistance 16S rDNA Genomic library |
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