Identification of a RAPD marker linked to a brown planthopper resistance gene in rice |
| |
Authors: | Yong-Hee Jeon Sang-Nag Ahn Hae-Chune Choi Tae-Ryong Hahn Huhn-Pal Moon |
| |
Institution: | (1) Rice Breeding Division, National Crop Experiment Station, RDA, Suweon, 441–100, Korea;(2) Department of Genetic Engineering, KyungHee University, c[Yongin, 449-900, Korea |
| |
Abstract: | We report the tagging of a brown planthopper (BPH) resistance gene (Bph–1) in rice using RAPD and RFLP markers. The Korean
rice variety ‘Gayabyeo’ has dominant duplicate genes including Bph–1 conferring resistance to biotype 1 of BPH. Bulked segregant
RAPD analysis was employed for rapid identification of DNA markers linked to resistance genes. For tagging these two genes,
an F2F3 population from a ‘Gayabyeo’ × ‘Nagdongbyeo’ cross was developed and evaluated for BPH resistance. Three bulked DNAs
from two groups of homozygous BPH resistant (each for Bph–1 and the other unknown gene) and homozygous susceptible F2 plants
were analyzed by RAPD using 140 random oligomers. One primer, OPD–7 yielded a 700-bp fragment that was present in Gayabyeo
and resistant F2 plants (homozygous for Bph-1 locus) but absent in Nagdongbyeo and susceptible F2 plants. Cosegregation of
this marker with Bph-1 was verified using an F2 population segregating for Bph-1. Chromosomal regions surrounding the Bph-1
were examined with additional RFLP and microsatellite markers on chromosome 12 to define the location of the RAPD marker and
Bph-1. Use of this RAPD marker could facilitate early selection of resistant lines for BPH.
This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
Keywords: | Bph-1 linkage analysis mapping RAPD RFLP rice |
本文献已被 SpringerLink 等数据库收录! |
|