响应面法优化磷酸化改性花生分离蛋白－多肽膜的制备工艺

    为了优化磷酸化改性花生分离蛋白-多肽膜制备工艺条件,在单因素基础上,通过响应面Box-Benhnken进行实验设计。结果表明,最优工艺参数：蛋白浓度8%、pH值8.2、甘油百分含量（占蛋白）13.4%、黄原胶百分含量（占蛋白）1%、时间60min、温度69℃、超声波功率270W、超声波频率28kHz、多肽溶液的浓度61mg/mL;此工艺条件下,膜厚度、吸水率和透光率理论预测值分别为86μm、41.9%和53.6%,验证实验值分别为88±2μm、43.1±1.2%和52.4±1.5%,两者的差值分别为2.33%、2.86%和2.24%,说明响应面二次模型的拟合良好;磷酸化改性花生分离蛋白-多肽膜的抗拉强度9.62MPa、断裂延伸率101.68%、溶解性47.69%、水蒸气透过率6.95 g•m-2· h-1等功能性质和DPPH自由基清除活性IC50值7.70 mg·mL-1、羟自由基清除活性IC50值5.98 mg·mL-1、超氧阴离子自由基清除活性IC50值4.20 mg·mL-1、铁离子螯合力活性IC50值3.79 mg·mL-1、铜离子螯合力活性IC50值13.61 mg·mL-1、脂质过氧化抑制活性IC50值8.62 mg·mL-1、铁还原力IC50值13.93mg·mL-1、钼还原力IC50值5.49mg·mL-1等抗氧化活性较磷酸化改性花生分离蛋白膜有所改善。本研究结果为磷酸化改性花生分离蛋白在蛋白膜方面的应用提供一种新途径。

Optimization of phosphorelated peanut protein isolate – polypeptide films preparation by response surface methodology #br#

     order to optimize the preparation process conditions of phosphorelated peanut protein isolate - polypeptide films, experiment was carried out with the response surface Box-Benhnken experiment, on the basis of single factor experiments. The results showed that the optimum conditions obtained were protein concentration, pH value, glycerol percentage content of protein, xanthan gum percentage content of protein, time, temperature, ultrasonic power, ultrasonic frequency, polypeptide concentration of 8%, 8.2, 13.4%, 1%, 60min, 69℃, 270w, 28kHz, 61mg/mL, respectively. Under the optimum conditions, the theoretical predictive values of film thickness, water absorption and light transmittance were 86μm, 41.9% and 53.6%, respectively. The results of verification experiment showed that film thickness, water absorption rate and light transmittance rate could reach 88±2μm, 43.1±1.2% and 52.4±1.5%, respectively. The differences between experimental and theoretical values were 2.33%, 2.86% and 2.24%, respectively. This suggested the response surface quadratic model fitted well. The functional properties were better than those of phosphorelated modified peanut protein isolate films, and these functional properties included tensile strength of 9.62 MPa, elongation of 101.68 %, solubility of 47.69 %, water vapor permeability of 6.95 g•(m2·h)-1 and antioxidant activities including DPPH free radical scavenging activity of IC50 7.70 mg·mL-1, hydroxy free radical scavenging activity of IC50 5.98 mg·mL-1, superoxide anion free radical scavenging activity of IC50 4.20 mg·mL-1, iron ion chelating activity of IC50 3.79 mg·mL-1, copper ion chelating activity of IC50 13.61 mg·mL-1, lipid peroxidation inhibition activity of IC50 8.62 mg·mL-1, iron reducing power of IC50 13.93 mg·mL-1, molybdenum reducing power of IC50 5.49 mg·mL-1 of phosphorelated peanut protein isolate - polypeptide films. The results of this study provided a new way for the application of phosphorelated peanut protein isolate.