大豆GmCYP82C4基因克隆与表达分析

细胞色素P450 CYP82家族基因为双子叶植物特有,参与生长代谢和逆境生理调控。为了探究大豆P450 CYP82的基因功能,克隆大豆P450同源基因GmCYP82C4,得到该基因编码区1584bp,它编码527个氨基酸,编码的蛋白质具有保守的血红素结合域。生物信息学分析表明,GmCYP82C4蛋白与大豆中GmCYP82A3同源性最高,其次为豌豆的PsCYP82A1。亚细胞定位结果显示,GmCYP82C4定位于细胞质膜。实时荧光定量结果表明,GmCYP82C4在大豆幼苗根中表达量最高,能够响应低温、伤害和大豆疫霉胁迫,并受到脱落酸、茉莉酸甲酯、乙烯和水杨酸信号调控。由此推测GmCYP82C4可能通过激素信号途径调控大豆对胁迫的响应。

Cloning and expression analysis of GmCYP82C4 gene in soybean

Cytochrome P450 CYP82 is in a unique gene family in dicotyledonous plants. To better undrstand its important roles in plant growth and response to biotic and abiotic stresses, soybean gene GmCYP82C4 was homologously cloned and identified. The gene was 1584 bp long, encoding 527 amino acids with a conservative heme-binding domain. Bioinformatics showed that GmCYP82C4 protein was homology with PsCYP82A1 in pea. Subcellular localization showed that GmCYP82C4 was located in plasma membrane. Real-time fluorescence quantitative PCR showed that GmCYP82C4 expression in roots of soybean seedlings was the highest. GmCYP82C4 expression was induced by low temperature, wound (cut) and Phytophthora sojae infection respectively. GmCYP82C4 was responsive to plant hormones as ABA, MeJA (methyl jasmonate), ethylene and SA (salicylic acid). These results suggested that GmCYP82C4 might regulate the response of soybean to stress through hormonal signaling pathway.