ISSR标记揭示我国向日葵列当群体的遗传多样性

向日葵列当是目前严重危害我国向日葵生产的寄生性种子植物。为了明确我国不同地区向日葵列当的亲缘关系，本试验利用ISSR标记对采自我国不同省份的96份向日葵列当样本进行了群体遗传多样性的研究。结果表明：从100条ISSR引物中筛选出多态性高、重复性好的12条引物用于群体遗传分析；以供试的列当DNA为模板，利用上述筛选到的引物进行PCR 扩增，共扩增出147 条带，其中90 条具有多态性，多态性条带的百分率为61.2%。不同地区采集的列当群体的Shannon信息指数和Nei′s多样性指数均随着群体样本数量的增加而增大，其中内蒙古地区和新疆地区向日葵列当的多态性相对较高，Shannon信息指数分别为0.5560和0.5067。遗传聚类结果表明6个不同省区的向日葵列当可被聚成两个亚组，其中山西、河北和陕西聚成一个亚组，吉林、新疆和内蒙古聚成另外一个亚组；河北和陕西来源的列当样本亲缘关系最近，而新疆与河北两地的列当样本亲缘关系最远。

Application of ISSR markers to reveal the genetic diversity of sunflower broomrape in China

Sunflower broomrape is parasitic seed plant seriously endangering the sunflower industry in China. To clarify the genetic relationship of sunflower broomrape in different regions of China, ISSR markers were used on population genetic diversity of 96 sunflower broomrape samples from different provinces in China. Results showed 12 primers with high polymorphism and good reproducibility screened from 100 ISSR primers. A total of 147 bands were amplified by PCR, of which 90 were polymorphic. The percentage of polymorphic bands was 61.2%. Shannon information index and Nei′s diversity index of broomrape population from different locations were both increased with the increase of population sample size. Among them, broomrapes form Inner Mongolia and Xinjiang provinces showed higher polymorphism. Their Shannon indices were 0.5560 and 0.5067 respectively. Genetic clustering re? sults showed that the samples from 6 different provinces could be divided into 2 sub-groups. Shanxi, Hebei and Shaanxi samples were clustered into one sub-group; and those from Jilin, Xinjiang and Inner Mongolia were clus? tered into another sub-group. Broomrape samples between Hebei and Shaanxi had the closest relationship, while samples from Xinjiang and Hebei had the furthest relationship.