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| 生长素和细胞分裂素对悬浮培养人参细胞生长和皂苷合成的影响 | |
| 引用本文： | 修景润,廉美兰,朴成日,李慧娟,朴炫春. 生长素和细胞分裂素对悬浮培养人参细胞生长和皂苷合成的影响[J]. 延边大学农学学报, 2011, 33(2): 83-88 |
| 作者姓名： | 修景润 廉美兰 朴成日 李慧娟 朴炫春 |
| 作者单位： | 1. 延边大学长白山生物功能因子省部共建教育部重点实验室,吉林延吉133002;延边朝鲜族自治州农业科学院,吉林龙井133400 2. 延边大学长白山生物功能因子省部共建教育部重点实验室,吉林延吉,133002 3. 延边朝鲜族自治州农业科学院,吉林龙井,133400 |
| 基金项目： | 延边大学“211工程”3期建设项目；中韩合作项目 |
| 摘 要： | 为探明人参细胞悬浮过程中植物生长调节剂对细胞生长和皂苷合成的影响,调查IBA、NAA、2,4-D单独使用及IBA与BA和IBA与KT混合使用的效果,结果表明,在2,4-D处理中细胞生物量虽然高于IBA和NAA,但总皂苷含量低于IBA和NAA处理;在生长素单独使用处理中,IBA 7 mg/L处理的皂苷生产量最高（72.9 mg/L）.当IBA 7 mg/L与BA或KT混合使用时,促进细胞生长和皂苷合成,其中IBA 7 mg/L＋BA0.5 mg/L和IBA 7 mg/L＋KT 0.5 mg/L处理中,皂苷生产量明显提高,分别达到115.92和120.98 mg/L.因此,在进行人参细胞悬浮培养时,IBA 7 mg/L与BA 0.5 mg/L或KT混用可高效生产人参皂苷,为食品及药品生产提供安全的原料. |
| 关 键 词： | 人参细胞 悬浮培养 植物生长调节剂 |
Effect of auxins and cytokinins on cell growth and ginsenoside production of Panax ginseng during suspension culture |
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| XIU Jing-run,LIAN Mei-lan,PIAO Cheng-ri,Li Hui-juan,PIAO Xuan-chun. Effect of auxins and cytokinins on cell growth and ginsenoside production of Panax ginseng during suspension culture[J]. Journal of Agricultural Science Yanbian University, 2011, 33(2): 83-88 | |
| Authors: | XIU Jing-run LIAN Mei-lan PIAO Cheng-ri Li Hui-juan PIAO Xuan-chun |
| Affiliation: | XIU Jing-run1,2,LIAN Mei-lan1,PIAO Cheng-ri2,Li Hui-juan1,PIAO Xuan-chun1 (1.Key Laboratory of Nature Resource of Changbai Mountain & Functional Molecular(Yanbian University),Ministry of Education,Yanji Jilin 133002,China,2.Academy of Agricultural Sciences of Yanbian,Longjing Jilin 133400,China) |
| Abstract: | To select appropriate plant growth regulators during cell suspension culture of Panax ginseng,the effect of IBA,NAA,2,4-D,and combination of IBA with BA or KT on cell growth and ginsenoside production were investigated.The results showed that the better cell growth was observed in MS medium supplemented with 1.0 mg/L 2,4-D while higher ginsenoside contents were analyzed in IBA and NAA treatments,the maximum ginsenoside productivity(72.9 mg/L) was found in 7 mg/L IBA.In addition,the combination of IBA with d... |
| Keywords: | Panax ginseng cell suspension culture plant growth regulator |
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