In vitro isolation of Neospora caninum from a stillborn calf in the UK. |
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| Authors: | H C Davison F Guy A J Trees C Ryce J T Ellis A Otter M Jeffrey V R Simpson J J Holt |
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| Affiliation: | Veterinary Parasitology, Liverpool School of Tropical Medicine/Faculty of Veterinary Science, Pembroke Place, Liverpool, L3 5QA, UK. |
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| Abstract: | Neospora caninum was isolated in Vero cell culture from the brain of a stillborn calf. This isolate (designated NC-LivB1) is the first to be obtained from cattle in the United Kingdom and was confirmed as N. caninum by immunofluorescence with specific antibodies and by internal transcribed spacer 1 (ITS1) sequence analysis. Differences were found between NC-LivB1, other bovine isolates and canine isolates of N. caninum and closely related protozoal parasites, using random amplified polymorphic DNA polymerase chain reaction (RAPD - PCR) techniques. |
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