花叶良姜离体再生体系的建立

【目的】建立花叶良姜高频高效离体再生体系,为其种苗周年规模化生产提供新方法。【方法】以花叶良姜种子为材料,开展无菌萌发、愈伤组织诱导、愈伤组织分化及生根培养研究。【结果】花叶良姜种子依次经75%乙醇处理60 s、0.1%升汞处理10 min、5%次氯酸钠处理3 min,污染率仅5.00%,萌发率为75.83%;适宜花叶良姜愈伤组织诱导的培养基为MS+1.50 mg/L 6-BA+0.30 mg/L 2,4-D,适宜花叶良姜愈伤组织分化的培养基为MS+2.00 mg/L TDZ+0.10 mg/L 2,4-D,分化系数达10.03;花叶良姜组培苗最佳生根培养基为1/2MS+1.00 mg/L ABT1号生根粉,生根率为98.00%。【结论】通过愈伤组织途径可建立花叶良姜的离体再生体系。

Establishment of in vitro propagation technology of Alpinia zerumbet Variegata

Objective]The present study was conducted to establish high-efficiency and high-frequency in vitro re-generation system of Alpinia zerumbet Variegata,in order to provide a new method for annual large-scale production of A. zerumbet seedling. [Method]Using A. zerumbet seeds as materials,the experiment on aseptic budding,callus induction, callus differentiation and rooting was carried out. [Result]Results showed that, after A. zerumbet seeds were disinfected with 75%ethanol for 60 s,then 0.1%mercury chloride for 10 min,finally 5%sodium hypochlorite for 3 min,contamination rate was only 5.00%,and germination rate was 75.83%. Suitable medium for inducing callus was MS+1.50 mg/L 6-BA+0.30 mg/L 2,4-D,suitable medium for callus differentiation was MS+2.00 mg/L TDZ+0.10 mg/L 2,4-D,differentiation coef-ficient was up to 10.03. Furthermore,optimum medium for rooting was 1/2MS+1.00 mg/L ABT1 rooting powder,rooting rate was 98.00%.[Conclusion]In vitro regeneration system of A. zerumbet can be established by the way of callus culture.