Using an Arabidopsis oligonucleotide microarray to analyze the secretory pathway genes' Response to TuMV infection in Brassica rapa |
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| Institution: | 1. Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil;2. Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Brasília, DF, Brazil;3. Embrapa Hortaliças, Brasília, DF, Brazil;4. Universidad de Cordoba-Cei A3, Cordoba, Spain;1. Integrated Science Division, College of Health Sciences, University of Bahrain, Manama, Bahrain;2. Al Jawhara Centre for Molecular Medicine and Inherited Disorders, Arabian Gulf University, Bahrain;3. Department of Biology College of science, University of Bahrain, Bahrain;4. Proteomics Unit, Stem Cell & Tissue Re-Engineering Program, King Faisal Specialist Hospital and Research Centre (KFSH&RC), P.O. Box 3354, Riyadh 11211, Saudi Arabia;5. Department of Biochemistry, College of Medicine and Medical Sciences (CMMS), Arabian Gulf University (AGU), Manama, Bahrain;1. Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, 62214 Cuernavaca, Morelos, Mexico;2. Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad 565, Chamilpa, 62210 Cuernavaca, Morelos, Mexico |
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| Abstract: | Previous evidence suggested the Turnip mosaic virus viral ribonuclear protein complex, comprising the viral RNA, moved to the plasmodesmata via the secretory pathway. We performed a global analysis of gene expression in Brassica rapa and identified a complex array of changes affecting pathogen response. Thirty-eight secretory pathway genes were identified. The quantitative real-time polymerase chain reaction results were consistent with microarray analysis. Combined with previous studies, we speculated that the secretory pathway participates in viral replication and movement. Exploring the precise function of the genes identified in this analysis will offer new insights into viral defense in B. rapa. |
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