Identification of genes associated with phytoplasma resistance through suppressive subtraction hybridization in Chinese jujube |
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| Affiliation: | 1. Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding 071001, China;2. College of Life Science, Agricultural University of Hebei, Baoding 071000, China;3. Department of Molecular and Cellular Biochemistry and Center for Structural Biology, University of Kentucky, Lexington, Kentucky 40536;4. Institute of Plant Biology, University of Zürich, CH-8008, Zürich, Switzerland;5. Institute of Agricultural Sciences, Eidgenössische Technische Hochschule (ETH) Zürich, CH-8092, Zürich, Switzerland;1. State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Ministry of Education, Tianjin 300457, China;2. College of Food Science and Pharmacy, Xinjiang Agricultural University, Xinjiang 830052, China;3. College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China;1. Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;2. Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;1. Department of Agroecology, Faculty of Science and Technology, Aarhus University, Forsøgsvej 1, Slagelse, DK-4200, Denmark;2. Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom |
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| Abstract: | Jujube witches' broom (JWB) is a destructive disease for Chinese jujube caused by phytoplasma. A suppression subtractive hybridization library of resistant cultivar ‘Xingguang’ was constructed under phytoplasma stress to identify genes related to JWB resistance. 77 of 200 unique expressed sequence tags had significant sequence homologies and were classified into 10 functional groups. The most abundant group was disease/defense (20.8%), which was consistent with the phytoplasma stress. These differentially expressed genes provide the groundwork for addressing the plant–phytoplasma interaction. Meanwhile, the expression of five selected genes (TLP, PR10, HSP70, ERF, kinase-related protein) was confirmed to upregulate at different infection periods. |
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| Keywords: | Chinese jujube Jujube witches' broom disease Phytoplasma Suppression subtractive hybridization |
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