| 茶树类黄酮3-O-葡萄糖基转移酶基因的克隆和表达分析 |
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| 引用本文： | 王晓帆,田艳维,王云生,高丽萍,夏涛.茶树类黄酮3-O-葡萄糖基转移酶基因的克隆和表达分析[J].茶叶科学,2012,32(5):411-418. |
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| 作者姓名： | 王晓帆 田艳维 王云生 高丽萍 夏涛 |
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| 作者单位： | 1. 安徽农业大学 教育部茶叶生物化学与生物技术重点实验室,安徽 合肥 230036; 2. 安徽农业大学生命科学学院,安徽 合肥 230036 |
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| 摘 要： | 以茶树叶片为材料,结合同源克隆方法和RACE技术,克隆了1条UFGT基因,命名为CsUFGT。该基因cDNA全长为1526bp,ORF长1380bp,编码459个氨基酸,推测等电点5.96,推测分子量为49.486kDa。该基因编码的蛋白质与葡萄UFGT（P51094.2）的一致性为59%,相似性为75%,其C-端含有植物UGT家族成员特有PSPG基序。荧光实时定量PCR分析表明,该基因在茶树根茎叶中均表达,在第4叶表达量最高,根和茎中表达量较低。
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| 关 键 词： | 茶树 类黄酮3-O-葡萄糖基转移酶 基因克隆 表达分析 |
| 收稿时间： | 2011-10-13 |
The Gene Cloning and Expression Analysis of UFGT in Tea Plant [Camellia sinensis (L.) O.Kuntze] |
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| WANG Xiao-fan
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TIAN Yan-wei
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WANG Yun-sheng
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GAO Li-ping
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XIA Tao.The Gene Cloning and Expression Analysis of UFGT in Tea Plant [Camellia sinensis (L.) O.Kuntze][J].Journal of Tea Science,2012,32(5):411-418. |
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| Authors: | WANG Xiao-fan TIAN Yan-wei WANG Yun-sheng GAO Li-ping XIA Tao |
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| Institution: | 1. Key Lab of Tea Biochemistry and Biotechnology, Ministry of Education, Anhui Agricultural University, Hefei 230036, China; 2. School of Biology Science, Anhui Agricultural University, Hefei 230036, China |
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| Abstract: | A glucosyltransferase gene UDP-flavonoid 3-O-glucosyl transferase was isolated from tea plant Camellia sinensis(L.) O.Kuntze] and named CsUFGT.CsUFGT has 1 526 bp full length with open reading frame of 1380 bp which encodes 459 amino acids.The corresponding protein CsUFGT,with predicted molecular mass 49.486 kDa and predicted isoelectric point 5.96,shares 59% identity and 75% similarity with UFGT(P51094.2) in Vitis vinifer.CsUFGT includes a PSPG signal motif of typical plant glucosyltransferase.qRT-PCR analysis showed that the gene expressed in all tissues of tea plant Camellia sinensis(L.) O.Kuntze],and had high expression in the fourth leaf and low expression level in root and stem. |
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| Keywords: | Camellia sinensis flavonoid 3-O-glucosyl transferase gene cloning expression analysis |
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