| 麻类韧皮纤维细胞骨架制备条件优化研究 |
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| 引用本文： | 冷超,王克臣,周亚东,苏钰,李明. 麻类韧皮纤维细胞骨架制备条件优化研究[J]. 东北农业大学学报, 2010, 41(3) |
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| 作者姓名： | 冷超 王克臣 周亚东 苏钰 李明 |
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| 作者单位： | 东北农业大学农学院,哈尔滨,150030;哈尔滨学院,哈尔滨,150080;哈尔滨学院,哈尔滨,150080;东北农业大学农学院,哈尔滨,150030 |
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| 基金项目： | 黑龙江省博士后资金资助(LRB08-491) |
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| 摘 要： | 试验以亚麻、大麻和青麻细胞壁加厚的韧皮纤维为材料,进行细胞骨架制备条件优化研究。即在普通考马斯亮蓝R250染色基础之上,对1%Triton X-100抽提非骨架蛋白时间进行了优化。结果表明,在37℃恒温箱中用1%Triton X-100处理材料30 min,纤维细胞骨架清晰可见。试验为进一步研究细胞骨架系统和纤维素微纤丝如何在细胞壁中沉积等奠定了基础。
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| 关 键 词： | 细胞骨架 纤维细胞 亚麻 大麻 青麻 |
Optimization preparation conditions of fibre cell cytoskeleton morphology of bast fibre crops |
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| LENG Chao,WANG Kechen,ZHOU Yadong,SU Yu,LI Ming. Optimization preparation conditions of fibre cell cytoskeleton morphology of bast fibre crops[J]. Journal of Northeast Agricultural University, 2010, 41(3) |
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| Authors: | LENG Chao WANG Kechen ZHOU Yadong SU Yu LI Ming |
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| Affiliation: | 1.College of Agriculture;Northeast Agricultural University;Harbin 150030;China;2.Harbin Uni-versity;Harbin 150080;China |
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| Abstract: | The bast fibres with the thicken cell wall of flax, hemp and abutilon were used as test materials to study the cytoskeleton morphology, and to optimize the time of non-matrix proteins by 1% Triton X-100 with coomassie brilliant blue R250 staining. The results showed that cytoskeleton of the fibre cell was clearly visible through 30 min treatment by 1% Triton X-100 at 37℃. This experiment established the foundation for studying cytoskeleton system and how the cellulose microfibril deposied in the cell wall in the future. |
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| Keywords: | cytoskeleton fibre cell flax hemp abutilon |
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