一株纤维素降解菌的筛选与鉴定

从落叶覆盖的腐殖层土壤中富集、分离得到12株纤维素降解菌,从中筛选出一株高效降解菌(X10),研究其最适宜的培养条件为:配制以葡萄糖+微晶纤维素(1∶1)为碳源、以蛋白胨+牛肉膏(1∶1)为氮源的培养基,pH值7.5,30℃下培养40 h,测得纤维素降解酶酶活可达到67.44 U。通过对其16s rRNA测序鉴定,该菌与氧化微杆菌(Microbacterium oxydans)有99%的同源性。纤维素降解菌的选育可为高效生产纤维素酶提供新的菌种来源。

Screening and Identification of Cellulose Degradation Bacteria

12 cellulose-degrading bacterias were enriched and isolated from the leaves of the humus layer covering the soil and a high-degrading bacteria(X10)was selected from these.The optimal culture conditions of the bacteria were as follows:took the culture medium containing glucose+micrite cellulose(1∶1)as carbon sources,peptone+beef(1∶1)as nitrogen sources,pH 7.5,under 30 ℃,with the culture time was 40 h,the cellulose-degrading enzyme activity was up to 67.44 U.Through its 16s rRNA sequencing,the homology of bacteria and Microbacterium oxydans were 99%.The selection of cellulose-degrading bacteria provided a new source of bacteria for the efficient production of cellulase.