| 广东西瓜上检测到甜瓜黄斑病毒 |
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| 引用本文： | 刘勇,吴竹妍,黎园,饶雪琴. 广东西瓜上检测到甜瓜黄斑病毒[J]. 植物检疫, 2011, 0(6) |
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| 作者姓名： | 刘勇 吴竹妍 黎园 饶雪琴 |
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| 作者单位： | 华南农业大学资源环境学院; |
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| 基金项目： | 广东省自然科学基金(07300670) |
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| 摘 要： | 在广东发现了可能被番茄斑萎病毒属(Tospovirus)病毒侵染的西瓜,采用ELISA和RT-PCR法对该西瓜病样进行了检测,西瓜病叶粗汁液不与番茄斑萎病毒(Tomato spotted wilt virus,TSWV)和西瓜银斑驳病毒(Watermelon silver mottle virus,WSMoV)的血清发生反应;利用引物J13/UHP通过RT-PCR可以扩增出约1400 bp的基因片段,该片段包括一个840 bp的核衣壳蛋白ORF,其推导的氨基酸序列与已报道的Melon yellow spot virus(MYSV)NP基因氨基酸序列的同源率都为99%,进化树分析表明侵染广东西瓜的病毒(命名为MYSV-GZ)属于Tospovirus的MYSV血清组。
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| 关 键 词： | 甜瓜黄斑病毒 番茄斑萎病毒属 西瓜 RT-PCR |
Detection of Melon yellow spot virus on watermelon in Guangdong |
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| Liu Yong,Wu Zhuyan,Li Yuan,Rao Xueqin. Detection of Melon yellow spot virus on watermelon in Guangdong[J]. Plant Quarantine, 2011, 0(6) |
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| Authors: | Liu Yong Wu Zhuyan Li Yuan Rao Xueqin |
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| Affiliation: | Liu Yong,Wu Zhuyan,Li Yuan,Rao Xueqin(College of Natural Resources and Environment,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | A watermelon sample possibly infected by tospovirus in Guangdong was detected by ELISA and RT-PCR.Crude extracts from yellow spot watermelon leaves were not react with antisera of Tomato spotted wilt virus(TSWV) and Watermelon silver mottle virus(WSMoV) by ELISA.When they were tested by RT-PCR using primer pair J13/UHP,about 1400bp fragment was amplified,which contained 840bp ORF of nucleocapsid protein(NP).Sequence analysis showed that the NP shared 99% identity at the amino acid level with those reported ... |
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| Keywords: | Melon yellow spot virus(MYSV) Tospovirus watermelon RT-PCR |
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