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土壤微生物多样性研究方法综述
引用本文:LIU Bing-Ru,JIA Guo-Mei,CHEN Jian,WANG Gang. 土壤微生物多样性研究方法综述[J]. 土壤圈, 2006, 16(1): 18-24. DOI: 10.1016/S1002-0160(06)60021-0
作者姓名:LIU Bing-Ru  JIA Guo-Mei  CHEN Jian  WANG Gang
作者单位:Key Laboratory of Arid and Grassland Agroecology at Lanzhou University, Ministry of Education, Lanzhou 730000 (China). E-mail: liubr02@st.lzu.edu.cn;Key Laboratory of Arid and Grassland Agroecology at Lanzhou University, Ministry of Education, Lanzhou 730000 (China). E-mail: liubr02@st.lzu.edu.cn;Key Laboratory of Arid and Grassland Agroecology at Lanzhou University, Ministry of Education, Lanzhou 730000 (China). E-mail: liubr02@st.lzu.edu.cn;Key Laboratory of Arid and Grassland Agroecology at Lanzhou University, Ministry of Education, Lanzhou 730000 (China). E-mail: liubr02@st.lzu.edu.cn;School of Life Science, Lanzhou University, Lanzhou 730000 (China)
基金项目:project supported by the Key Research Program of Guangdong Province (No. 2002C20703) and the Key Research Program of the Forestry Administration of Guangdong Province (No. 2002-12).
摘    要:Soil microorganisms play a central role in decomposing organic matter, in determining the release of mineral nutrients, and in nutrient cycling. Recently, extensive studies have focused on soil microbial diversity. However, understanding the diversity of this complex microbial community in the soil environment is a challenging task. Thus, it is important to master and comprehend appropriate methods for studying soil microbial diversity. Concepts of soil microbial diversity and major methods of study are briefly introduced in this paper. Then, the application of biochemical-based and molecular-based techniques in this area, and their advantages and disadvantages are evaluated. Based on recent related research, perspectives for studying microbial diversity in soils are presented.

关 键 词:广东 东莞市 土壤化学 总有机碳 空间变化
收稿时间:2005-08-17
修稿时间:2005-12-09

A review of methods for studying microbial diversity in soils
LIU Bing-Ru,JIA Guo-Mei,CHEN Jian and WANG Gang. A review of methods for studying microbial diversity in soils[J]. Pedosphere, 2006, 16(1): 18-24. DOI: 10.1016/S1002-0160(06)60021-0
Authors:LIU Bing-Ru  JIA Guo-Mei  CHEN Jian  WANG Gang
Affiliation:Key Laboratory of Arid and Grassland Agroecology at Lanzhou University, Ministry of Education, Lanzhou 730000 (China). E-mail: liubr02@st.lzu.edu.cn;Key Laboratory of Arid and Grassland Agroecology at Lanzhou University, Ministry of Education, Lanzhou 730000 (China). E-mail: liubr02@st.lzu.edu.cn;Key Laboratory of Arid and Grassland Agroecology at Lanzhou University, Ministry of Education, Lanzhou 730000 (China). E-mail: liubr02@st.lzu.edu.cn;Key Laboratory of Arid and Grassland Agroecology at Lanzhou University, Ministry of Education, Lanzhou 730000 (China). E-mail: liubr02@st.lzu.edu.cn;School of Life Science, Lanzhou University, Lanzhou 730000 (China)
Abstract:Soil microorganisms play a central role in decomposing organic matter, in determining the release of mineral nutrients, and in nutrient cycling. Recently, extensive studies have focused on soil microbial diversity. However, understanding the diversity of this complex microbial community in the soil environment is a challenging task. Thus, it is important to master and comprehend appropriate methods for studying soil microbial diversity. Concepts of soil microbial diversity and major methods of study are briefly introduced in this paper. Then, the application of biochemical-based and molecular-based techniques in this area, and their advantages and disadvantages are evaluated. Based on recent related research, perspectives for studying microbial diversity in soils are presented.
Keywords:land-use pattern  organic carbon content  organic carbon density  spatial variability
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