人参三醇型皂苷黑根霉发酵产物与人参皂苷的增强记忆活性对比

通过建立3种损伤性拟痴呆模型,即东莨菪碱致胆碱能损伤拟痴呆小鼠模型、40%乙醇损伤拟痴呆小鼠模型、氯霉素注射致痴呆小鼠模型,每日给予小鼠受试药物,通过跳台试验和被动回避试验对小鼠进行行为学检测,并对小鼠脑内胆碱能含量进行检测,对比人参三醇型皂苷的黑根霉发酵产物和部分人参皂苷的增强记忆活性。结果表明:人参三醇型皂苷黑根霉发酵产物3种损伤模型中均显示出增强记忆活性,且与对照组相比差异显著。人参三醇型皂苷黑根霉发酵产物组和人参皂苷Rg1组小鼠脑内乙酰胆碱转移酶(ChAT)活性明显高于空白对照组(P0.05),乙酰胆碱酯酶(AchE)活性低于空白对照组(P0.05),说明人参三醇型皂苷黑根霉发酵产物不仅能降低AchE活性,而且能增强ChAT活性,并且此活性与小鼠脑内的胆碱能系统具有一定关系。

Comparative Study on Effects of Fermentation Product of Proto-panaxatriol Ginsenoside by Rhizopus nigricans and Ginsenoside on En-hancing Memory

This article is a comparative study of effects of fermentation product of protopanaxatriol Ginsenoside by Rhizopus nigricans and ginsenoside on memorizing disorders of model mice with Alzheimer′s disease ( AD) , through the establishment of three kinds of traumatic dementia model, including Scopolamine-induced dementia cholinergic damage in mouse model, 40% ethanol-induced mouse model of dementia and chloramphenicol injection induced mouse model of dementia, mice were treated with test drugs by oral administration, then jumping test and passive avoidance test were carried out to evaluate behavioral detection, and content of ChAT of mice was detected. The results showed that the fermentation product group in the three kinds of mouse model of dementia is shown to enhance memory activity. Compared with the blank control group, activity of ChAT in mice′s brains of the fermentation product of protopanaxatriol Ginsenoside by Rhizopus nigricans group and ginsenoside Rg1 group was obviously higher ( P<0. 05) , and activity of AchE was obviously lower ( P<0. 05) . But there was no significant difference between ginsenoside Rg1/Rb1 group and the con-trol group. From this point of view, fermentation product of protopanaxatriol Ginsenoside by Rhizopus nigricans can not only reduce activity of AchE, but can also enhance activity of ChAT, which could be related to mouse brain cholinergic system.