喷雾干燥法制备饲用植酸酶制剂 |
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引用本文: | 张琪,朱庆锋,崔百元,孔谦,王志林,晏石娟. 喷雾干燥法制备饲用植酸酶制剂[J]. 吉林农业大学学报, 2016, 0(6): 750-753. DOI: 10.13327/j.jjlau.2016.3281 |
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作者姓名: | 张琪 朱庆锋 崔百元 孔谦 王志林 晏石娟 |
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作者单位: | 1. 广东省农业科学院农业生物基因研究中心,广州,510640;2. 广东省农业科学院动物科学研究所,畜禽育种国家重点实验室,广州510640 |
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基金项目: | 广东省农业攻关引导项目(2012B020305011),广东省科技计划项目(2011A020102009;2013A061401020),广东省农业领域科技计划项目(2013B020305005) |
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摘 要: | 以重组植酸酶(phyA-ABCD5F7)的毕赤酵母工程菌为植酸酶生产菌,经高密度发酵制备得到粗酶液。通过在粗酶液中添加Mg~(2+)和Zn~(2+)等金属离子以及海藻糖,可明显提高植酸酶的热耐受能力。以植酸酶的酶活回收率为评价指标,考察了进风温度、出风温度和保护剂对植酸酶酶活回收率的影响,确定最佳喷雾条件:保护剂5 g/L MgSO_4·7H_2O、2 g/L ZnSO_4·7H_2O和10 g/L海藻糖,进风温度为160℃,出风温度为70~75℃,在此条件下,植酸酶的酶活回收率达80%。
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关 键 词: | 植酸酶 喷雾干燥 保护剂 酶活 回收率 |
Preparation of Feed Phytase by Spray-drying |
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Abstract: | Recombinant pichia pastoris can secrete engineered phytase (phyA-ABCD5F7).After its high-density fermentation,crude enzyme liquid was prepared.Heat tolerance of phyA-ABCD5F7 can be improved via adding trehalose and metal ions such as Mg2+and Zn2+.The effects of inlet/outlet air temperature and several protectors were evaluated,according to their effects upon phytase activity recovery after spray-drying.The optimum spray-drying conditions were determined:5 g/L MgSO4 · 7H2O,2 g/L ZnSO4 · 7H2O and 10 g/L trehalose added as protectors,and 160 ℃/70 ~ 75 ℃ for inlet /outlet air temperature.Under such conditions,the recovery of phytase activity reached 80%. |
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Keywords: | phytase spray-drying protector enzyme activity recovery |
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