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| 花粉管通道法将Bt-CPTI双价抗虫基因转入玉米自交系的研究 | |
| 引用本文： | 李茫雪,张赫然,于晶,周子珊,苍晶,王振华.花粉管通道法将Bt-CPTI双价抗虫基因转入玉米自交系的研究[J].玉米科学,2010,18(1):29-33. |
| 作者姓名： | 李茫雪 张赫然 于晶 周子珊 苍晶 王振华 |
| 作者单位： | 1. 东北农业大学理学院,哈尔滨,150030 2. 东北农业大学生命科学学院,哈尔滨,150030 3. 东北农业大学农学院,哈尔滨,150030 |
| 基金项目： | 黑龙江省自然科学基金项目（C0305） |
| 摘 要： | 利用花粉管通道法将Bt-CPTI双价抗虫基因对优良玉米自交系进行遗传转化。采用组培和盆栽两种方法对大量t0代转基因种子进行卡那霉素抗性筛选，获得的卡那抗性植株进行PCR及PCR-Southern检测，初步证明外源目的基因已整合到玉米基因组中，PCR-Southern阳性率达到2.19%。 |
| 关 键 词： | 玉米 花粉管通道法 Bt-CPTII基因 |
| 收稿时间： | 2009/2/11 0:00:00 |
| 修稿时间： | 2009/3/26 0:00:00 |
Transferring Bt-CPTI Two Insect-insect Genes into Maize Inbred Lines by Pollen Tube Pathway |
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| LI Mang-xue,ZHANG He-ran,YU Jing,ZHOU Zi-shan,CANG Jing,WANG Zhen-hua.Transferring Bt-CPTI Two Insect-insect Genes into Maize Inbred Lines by Pollen Tube Pathway[J].Journal of Maize Sciences,2010,18(1):29-33. | |
| Authors: | LI Mang-xue ZHANG He-ran YU Jing ZHOU Zi-shan CANG Jing WANG Zhen-hua |
| Abstract: | Bt-CPTI gene was transformed into good maize inbred lines by pollen tube pathway. Tissue culture and potted plant were used in kanamycin-resistant screening among large number t0 generation of transgenic seeds. The kanamycin-resistant plants were detected by PCR and PCR-Southern and the positive rate of PCR-Southern achieved 2.19% which showed that the Bt-CPTI gene was integrated into maize genome. |
| Keywords: | Maize Method of pollen tube pathway Bt-CPTI gene |
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