| 鲁中肉羊SMAD1和ESR2基因多态性与产羔数关联分析 |
| |
| 引用本文： | 田志龙,潘林香,王金文,马琳,储明星.鲁中肉羊SMAD1和ESR2基因多态性与产羔数关联分析[J].中国草食动物科学,2019(2):13-16. |
| |
| 作者姓名： | 田志龙 潘林香 王金文 马琳 储明星 |
| |
| 作者单位： | 中国农业科学院北京畜牧兽医研究所农业部动物遗传育种与繁殖重点实验室;莱芜市嬴泰有机农业科技发展有限公司;山东省农业科学院畜牧兽医研究所 |
| |
| 基金项目： | 国家自然科学基金项目(31772580);国家肉羊产业技术体系专项(CARS-38);中国农业科学院科技创新工程(ASTIP-IAS13);中央级公益性科研院所基本科研业务费专项(Y2017JC24);农业科研杰出人才及其创新团队项目(农办人[2015]62号);国家万人计划科技创新领军人才项目(W02020274) |
| |
| 摘 要： | 为探究SMAD1、ESR2基因多态性与鲁中肉羊产羔数之间的关系,采用Sequenom MassARRAY誖SNP技术检测鲁中肉羊SMAD1、ESR2基因单核苷酸多态性,并与产羔数进行关联分析。结果表明:SMAD1基因g.12485895A>G存在AA、AG和GG基因型,基因型频率分别为0.05、0.45和0.50;ESR2基因g.73324006C>T存在CC和CT基因型,基因型频率分别为0.98和0.02。g.12485895A>G位点在鲁中肉羊表现为中度多态(0.25T位点为低度多态(PIC<0.25);卡方适合性检验表明,g.12485895A>G位点在鲁中肉羊处于哈代温伯格不平衡状态(P<0.05),g. 73324006C>T位点处于哈代温伯格平衡状态(P>0.05)。g.12485895A>G位点多态性与鲁中肉羊产羔数没有显著关联(P>0.05), g.73324006C>T位点多态性与鲁中肉羊产羔数显著关联(P<0.05)。综上可知,SMAD1基因g.12485895A>G位点和鲁中肉羊产羔数性状没有显著关联(P>0.05),ESR2基因g.73324006C>T位点对鲁中肉羊产羔数性状选育具有一定的指导意义。
|
| 关 键 词： | 绵羊 SMAD1 ESR2 分型 产羔数 |
Polymorphisms of SMAD1 and ESR2 Genes and Their Association with Litter Size in Luzhong Mutton Sheep |
| |
| Tian Zhilong,Pan Linxiang,Wang Jinwen,Ma Lin,Chu Mingxing.Polymorphisms of SMAD1 and ESR2 Genes and Their Association with Litter Size in Luzhong Mutton Sheep[J].China Herbivores,2019(2):13-16. |
| |
| Authors: | Tian Zhilong Pan Linxiang Wang Jinwen Ma Lin Chu Mingxing |
| |
| Institution: | (Key Laboratory of Animal Genetics,Breeding and Reproduction of Ministry of Agriculture,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Laiwu Yingtai Organic Agriculture Technology Development Co. LTD,Laiwu,Shandong 271114,China;Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan 250100,China) |
| |
| Abstract: | To elucidate the association of polymorphisms of SMAD1 and ESR2 genes and litter size in sheep.Luzhong mutton sheep were selected and Sequenom MassARRAY■SNP assay was applied to genotype SMAD1 and ESR2 genes.Then the association was analyzed between SMAD1 and ESR2 genes and litter size in Luzhong mutton sheep.The typing results showed that the SMAD1 gene g.12485895A>G had AA,AG and GG genotypes,and the genotype frequencies were 0.05,0.45 and 0.50,respectively.The ESR2 gene g.73324006C>T had CC and CT genotypes,and the genotype frequencies were 0.98 and 0.02,respectively.The result of population genetic analysis indicated that g.12485895A>G locus showed moderate polymorphism(0.25T locus showed low polymorphism(PIC<0.25);The χ^2 test indicated that the g.12485895A>G locus was not under Hardy-Weinberg equilibrium(P<0.05)and g.73324006C>T locus was under Hardy-Weinberg equilibrium(P>0.05).The results of association analysis indicated that the polymorphism of g.12485895A>G locus had no correlation with the litter size and the polymorphism of g.73324006C>T locus had correlation with the litter size(P<0.05).In summary,the SMAD1 gene g.12485895A>G has no significant impact on litter size in Luzhong mutton sheep and the g.73314006C>T locus might provide a basis for litter size trait selection in sheep. |
| |
| Keywords: | sheep SMAD1 gene ESR2 gene genotyping litter size |
| 本文献已被 维普 等数据库收录！ |
|
