应用Dot_ELISA技术检测草鱼出血病病毒的研究

报道了斑点免疫吸附ＥＬＩＳＡ（Ｄｏｔ－ＥＬＩＳＡ）快速检测草鱼出血病病毒（ＧＣＨＶ）的方法以及对提纯病毒、染毒细胞内病毒和病鱼组织的检测结果，并对该方法的灵敏度与ＳＰＡ－ＣｏＡ和常规ＥＬＩＳＡ法进行了系统的比较。结果表明，Ｄｏｔ－ＥＬＩＳＡ检出ＧＣＨＶ的最低含量为３ｐｇ，该灵敏度比ＳＰＡ－ＣｏＡ和常规ＥＬＩＳＡ法分别提高１０倍和２０倍，而且快速易行，是目前检测ＧＣＨＶ最为有效的方法，并可在草鱼出血病临床诊断和病毒疫苗质量鉴定等方面得到应用。

Studies on the detection of grass carp haemorrhagic virus by enzyme linked immunosorbent assay on nitrocellulose membrance (DOT-ELISA)

The present study desctibed a new technique of rapid detection for Grass Carp Haemorrhagic Virus(GCHV)with enzyme-linked immunosorbent assay on nitrocellulose membrance(DotELISA)The viruses, which existed in purified preparations as well as in extracts of infected cells and tissues, were detected respectively by the technique. Mean while, the sensitivity of the technique was estimated and the results showed that the smallest ammount to detect the purified virus was as little as3 Pg. In comparison with SPA coagglutination (SPA-CoA)test and ELISA, the sensitivity of DotELISA was at least ten times higher than that of SPA-CoA and twenty times than that of ELISA. Moreover,the test was rapid and easy to operate. It was indicated that the Dot-ELISA was the most valuable technique for the detection of GCHV at the present and could be further used in the diagnosis for haemorragic disease of Grass Carp and the quality identification in the viral vaccinne production.