| 番茄脱水素基因SlDHN2b 的克隆与表达分析 |
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| 引用本文： | 郭鹏,张士刚,金华,邹吉祥,董燕,姜国斌.番茄脱水素基因SlDHN2b 的克隆与表达分析[J].园艺学报,2012,39(10):2015-2022. |
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| 作者姓名： | 郭鹏 张士刚 金华 邹吉祥 董燕 姜国斌 |
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| 作者单位： | （1 大连民族学院环境与资源学院生物技术系，辽宁大连 116600；2 山东泰安市农业局，山东泰安 271018；3 辽宁林业职业技术学院，沈阳 110101） |
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| 基金项目： | 国家自然科学基金项目，辽宁省重点农业攻关计划项目，中央高校基本科研业务费资助项目 |
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| 摘 要： | 利用RT-PCR从番茄叶片中获得脱水素基因（dehydrins）的cDNA序列，命名为SlDHN2b。该基因开放阅读框1 140 bp，编码380个氨基酸。蛋白序列分析表明该蛋白高度亲水。Real-time PCR分析表明该基因受盐、脱水、ABA诱导表达。通过染色体步移技术获得SlDHN2b基因的启动子，PLACE数据库分析预测SlDHN2b启动子序列中含有多种逆境相关的作用元件。Northern杂交表明该基因在叶片中表达量最高，果实、花、茎中次之，根中最少。
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| 关 键 词： | 番茄 SlDHN2b 克隆 逆境 启动子 |
Cloning and Characterization of Dehydrins Gene SlDHN2b in Tomato |
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| GUO Peng,ZHANG Shi-gang,JIN Hua,ZOU Ji-xiang,DONG Yan,and JIANG Guo-bin.Cloning and Characterization of Dehydrins Gene SlDHN2b in Tomato[J].Acta Horticulturae Sinica,2012,39(10):2015-2022. |
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| Authors: | GUO Peng ZHANG Shi-gang JIN Hua ZOU Ji-xiang DONG Yan and JIANG Guo-bin |
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| Institution: | （1College of Environment and Resources，Dalian Nationalities University，Dalian，Liaoning 116600，China；2Tai’an Municipal Bureau of Agriculture，Tai’an，Shandong 271018，China；3Liaoning Forestry Vocational and Technical College，Shenyang 110101，China） |
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| Abstract: | In present study，a cDNA clone，designated SlDHN2b，was isolated from tomato by RT-PCR. The ORF of SlDHN2b was 1 140 bp and contained 380 amino acid residues. Protein sequence analysis showed that the SlDHN2b was high hydrophilic. Real-time PCR analysis indicated that mRNA accumulation of SlDHN2b was induced by salt stress，abscisic acid（ABA）and dehydration. Analysis of the promoter of PdEPF1 revealed the presence of stress-responsive elements by genome walking kit. Tissue-specific expression indicated that SlDHN2b was mainly expressed in leaves，weakly expressed in bud，flower，stem and the lowest in roots. Analysis of the promoter of SlDHN2b revealed the presence of stress-responsive elements. |
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| Keywords: | tomato SlDHN2b cloning abiotic stress promoter |
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