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柑橘日灼病果皮抗氧化代谢的变化
引用本文:万继锋,李娟,陈杰忠.柑橘日灼病果皮抗氧化代谢的变化[J].园艺学报,2012,39(10):2009-2014.
作者姓名:万继锋  李娟  陈杰忠
作者单位:(1 华南农业大学园艺学院,广州 510642;2 福建省农业科学院果树研究所,福州 350013;3 仲恺农业工程学院园艺园林学院,广州 510225)
基金项目:国家自然科学基金项目,广东省自然科学基金项目,广东省农业攻关项目,农业部行业科技子项目,广东省科技厅马圩贡柑中心专业镇公共服务平台创新提升建设项目
摘    要: 以砂糖橘、贡柑、红江橙和暗柳橙为试材,调查其田间日灼率和果实日灼后果皮抗氧化水平的变化。结果表明,不同品种田间日灼率不同;果实发生日灼后果皮组织中超氧阴离子自由基大量积累,脂氧合酶(LOX)活性显著增强,脂质过氧化产物丙二醛(MDA)含量显著上升,加剧细胞膜脂过氧化。超氧化物歧化酶(SOD)活性、抗坏血酸(AsA)和谷胱甘肽(GSH)含量均显著下降,过氧化物酶(POD)和多酚氧化酶(PPO)活性均显著增强,说明其抗氧化代谢失调。

关 键 词:柑橘  果实  日灼  抗氧化

Changes in Antioxidant Metabolism in the Fruit Pericarps of Citrus During Sunburn Development
WAN Ji-feng,LI Juan,and CHEN Jie-zhong.Changes in Antioxidant Metabolism in the Fruit Pericarps of Citrus During Sunburn Development[J].Acta Horticulturae Sinica,2012,39(10):2009-2014.
Authors:WAN Ji-feng  LI Juan  and CHEN Jie-zhong
Institution:(1College of Horticulture,South China Agricultural University,Guangzhou 510642,China;2Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;3Department of Horticulture,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)
Abstract:Fruit sunburn rates of four citrus varieties such as‘Shatangju’,‘Gonggan’,‘Hongjiang’sweet orange and‘Anliu’sweet orange were investigated. Fruits were used as materials to find out the changes of antioxidant level. The results showed that the fruit sunburn rates were different in different citrus varieties. After fruit sunburned,superoxide anion was significantly accumulated in pericarp,lipoxygenase(LOX)activity and malondialdehyde(MDA)content significantly increased,leading to aggravation of cell membrane lipid peroxidation. Correspondingly,superoxide dismutase(SOD)activity,ascorbic acid(AsA)and glutathione(GSH)contents decreased significantly,peroxidase(POD)and polyphenoloxidase(PPO)activities were significantly enhanced. These results indicated that the epidermal antioxidant metabolism in fruit was disordered after fruit sunburned.
Keywords:citrus  fruit  sunburn  antioxidant
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