Metabolism of amitrole in apple: II. Bound residues from mature fruits |
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Authors: | Bernd Schneider,Manfred Stock,Horst R. Schü tte,Klaus Schreiber,Johannes K ster,Ernst U. Kauß mann |
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Affiliation: | Bernd Schneider,Manfred Stock,Horst R. Schütte,Klaus Schreiber,Johannes Köster,Ernst U. Kaußmann |
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Abstract: | Significant radioactivity detected in mature fruits, harvested from apple trees (Malus domestica Borkh., cv. ‘Golden Delicious’ and ‘Gloster’) that were soiltreated with [3,5-14C]amitrole, remained in the insoluble plant material after exhaustive extraction. These bound residues were solubilized with a mixture of pectinases and cellulases. Thus, separation and characterization of carbohydrates and xenobiotic moieties released during this procedure became possible. A part of the radiolabel was incorporated into natural products, indicating degradation of the applied amitrole and reassimilation of [14C] carbon dioxide. |
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