兰花野百合花叶病的毒病原鉴定

 在日本东北大学农学研究所(仙台市),采集兰花野百合(Crotalaria sessiliflora L.)花叶病标样,经病毒提纯和蔗糖梯度密度分离,获得一种病毒分离物,该分离物的比重与CMV相近。经电镜鉴定和病组织超薄切片观察,该病毒的颗粒形态、大小及在细胞质和液胞中的病毒结晶体也与CMV相似,初步认为属CMV病毒组成员。但经琼脂免疫双扩散抗血清试验,该病毒的沉降线与PSV(落花生矮化病毒)的沉降线完全融合。还初步测定了寄主反应,也基本与PSV的寄主反应一致。因此,认定Crotalaria sessiliflara L.花叶病的毒病原是CMV病毒组的Peanut Stunt Virus(PSV)病毒(落花生矮化病毒)。

IDENTIFICATION OF VIRUS PATHOGEN OF CROTALARIA SESSILIFLORA MOSAIC

By purification of viruses and separation of sucrose density gradient we obtain an extracted virus from the sample of Crotalaria sessiliflora mosaic which is collected in the Agronomic Institute of the Japan Northeast University.The virus we found is similiar to CMV in gravity.And,by identifying through electronic microscopy and ultrathin sections of virus tissue,it is also similiar to CMV in shape,size of pellelt and crystal of cytoplasm and sap of vesicle.Therefore,we have an initial idea that the virus is in the same group of CMV.that the divi-Further more,in the experiment of agar immune response,we have observed ding line of settlement of the virus mixes together with that of PSV completely We have still a preliminary determination that the host reaction is in accord with that of PSV.Hence,we believe that the virus pathogen of Crotald-ria sessilifor mosaic is in the same group of PSV.