| 酵母双杂交技术筛选朊蛋白PrP23-231互作蛋白 |
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| 引用本文： | 宋博翠,陈志宝,万家余,高宏伟.酵母双杂交技术筛选朊蛋白PrP23-231互作蛋白[J].中国畜牧兽医,2010,37(8):34-36. |
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| 作者姓名： | 宋博翠 陈志宝 万家余 高宏伟 |
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| 作者单位： | （1.黑龙江八一农垦大学， 大庆 163319； 2.军事医学科学院军事兽医研究所， 长春 130062) |
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| 摘 要： | 利用酵母双杂交技术，以pSos-PrP23-231载体为诱饵质粒，筛选小鼠脑cDNA文库中能与朊蛋白PrP23-231相互作用的蛋白。通过一次筛选，三次验证，从小鼠脑cDNA文库中得到2个阳性克隆，通过BLAST序列比对得到2个候选基因序列，并对验证后的阳性克隆的外源性片段进行测序及同源性分析，得到的蛋白编码基因可能与朊蛋白存在相互作用，其作用可能与疯牛病发病机制有关。
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| 关 键 词： | 朊蛋白 酵母双杂交 蛋白互作 |
Screening Proteins Interacting with PrP23-231 Using Yeast Two-hybrid System |
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| SONG Bo-cui,CHEN Zhi-bao,WAN Jia-yu,GAO Hong-wei.Screening Proteins Interacting with PrP23-231 Using Yeast Two-hybrid System[J].China Animal Husbandry & Veterinary Medicine,2010,37(8):34-36. |
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| Authors: | SONG Bo-cui CHEN Zhi-bao WAN Jia-yu GAO Hong-wei |
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| Institution: | (1.Heilongjiang Bayi Agricultural University，Daqing 163319，China；2. Research Institute of Veterinary，Academy of Military Medical Sciences，Changchun 130062，China) |
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| Abstract: | The mouse leukocyte cDNA library was screened with pSos-PrP23-231 as bait plasmid by yeast two hybrid system,then the positive yeast clones were obtained. The candidate gene were confirmed by DNA sequencing and BLAST analysis in NCBI,the gene sequence were obtained with their coding protein interacting with pSos-PrP23-231,this candidate genes possibly involve with pathogenesis of BSE. |
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| Keywords: | prion yeast two-hybrid system protein interaction |
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