| 南芥菜花叶病毒的RT-LAMP检测试剂盒的研制 |
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| 引用本文： | 陈先锋,张吉红,崔俊霞,张慧丽,郭立新,余澍琼.南芥菜花叶病毒的RT-LAMP检测试剂盒的研制[J].植物保护学报,2013,40(2):189-190. |
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| 作者姓名： | 陈先锋 张吉红 崔俊霞 张慧丽 郭立新 余澍琼 |
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| 作者单位： | 宁波出入境检验检疫局,宁波,315012 |
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| 基金项目： | 宁波市科研项目(2011C50080),国家质检总局项目(2011IK181,2013IK279),宁波检验检疫局项目(甬K07-2011) |
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| 摘 要： | 南芥菜花叶病毒(Arabis mosaic virus,ArMV)是我国对外公布的检疫性有害生物,其寄主广泛,极易传播、蔓延1],因此,加强对该病毒的检测具有重要的意义.目前,针对ArMV的检测方法主要有DAS-ELISA和RT-PCR技术2].这两种方法在检测周期方面具有明显的优势,且操作简单,但对仪器的依赖性较强.环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)是一种新颖的核酸扩增方法,具有检测速度快、周期短、操作简单和灵敏度高等优点,同时LAMP无需特殊的仪器.本试验对ArMV的LAMP检测试剂盒进行了研制.
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| 收稿时间： | 2012/11/6 0:00:00 |
Detection of Arabis mosaic virus by RT-LAMP |
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| Chen Xianfeng,Zhang Jihong,Cui Junxi,Zhang Huili,Guo Lixin and Yu Shuqiong.Detection of Arabis mosaic virus by RT-LAMP[J].Acta Phytophylacica Sinica,2013,40(2):189-190. |
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| Authors: | Chen Xianfeng Zhang Jihong Cui Junxi Zhang Huili Guo Lixin and Yu Shuqiong |
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| Institution: | Ningbo Entry-Exit Inspection and Quarantine Bureau, Ningbo 315012, Zhejiang Province, China;Ningbo Entry-Exit Inspection and Quarantine Bureau, Ningbo 315012, Zhejiang Province, China;Ningbo Entry-Exit Inspection and Quarantine Bureau, Ningbo 315012, Zhejiang Province, China;Ningbo Entry-Exit Inspection and Quarantine Bureau, Ningbo 315012, Zhejiang Province, China;Ningbo Entry-Exit Inspection and Quarantine Bureau, Ningbo 315012, Zhejiang Province, China;Ningbo Entry-Exit Inspection and Quarantine Bureau, Ningbo 315012, Zhejiang Province, China |
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