| 蒺藜苜蓿小孢子不同发育时期花蕾形态与花药脱分化能力的研究 |
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| 引用本文： | 谷文英. 蒺藜苜蓿小孢子不同发育时期花蕾形态与花药脱分化能力的研究[J]. 中国农学通报, 2011, 27(20): 29-33 |
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| 作者姓名： | 谷文英 |
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| 作者单位： | 1. 扬州大学动物科学与技术学院,江苏扬州,225009
2. 中国农业科学院北京畜牧兽医研究所,北京,100193 |
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| 基金项目： | 北京市科技计划项目“牧草种质资源苗期抗逆性评价鉴定”(Z09090501040902) |
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| 摘 要： | 本研究以豆科模式植物蒺藜苜蓿为材料，研究其小孢子不同发育时期与花蕾形态的相关性，以及花药脱分化能力。结果表明：蒺藜苜蓿小孢子发育可分为单核早中期、单核靠边期和双核期,不同时期的小孢子与花蕾外部形态具有明显相关性。根据花蕾形态判断小孢子不同发育时期并进行培养，其中单核靠边期小孢子的花药愈伤组织诱导率最高，可达37.21%；单核早中期为4.82%，而双核期小孢子的花药无脱分化能力。这说明小孢子单核靠边期是蒺藜苜蓿花药培养的最佳时期，此时期的花蕾膨大，萼片绿色且比花瓣稍短。
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| 关 键 词： | 活性成 活性成 |
| 收稿时间： | 2010-12-15 |
| 修稿时间： | 2011-02-10 |
Studys on bud morphology of cytological development period of microspore and dedifferentiation capacity of anthers of Medicago truncatula |
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| Gu Wenying,Qi Xinmei,Pang Qiaoyu,Wei zhenwu,Gao Hongwen. Studys on bud morphology of cytological development period of microspore and dedifferentiation capacity of anthers of Medicago truncatula[J]. Chinese Agricultural Science Bulletin, 2011, 27(20): 29-33 |
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| Authors: | Gu Wenying Qi Xinmei Pang Qiaoyu Wei zhenwu Gao Hongwen |
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| Affiliation: | Gu Wenying 1,Qi Xinmei 1,Pang Qiaoyu 1,Wei zhenwu 1,Gao Hongwen 2 ( 1 College of Animal Science and Technology,Yangzhou University,Yangzhou Jiangsu 225009,2 The Animal Husbandry and Veterinary Institute of Beijing,Chinese Academy of Agricultural Science,Beijing 100193) |
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| Abstract: | In this experiment, Medicago truncatula plants were used to study the correlation between microspore development and bud morphology, dedifferentiation capacity of the anther was also determined. The results showed that microspore development of Medicago truncatula could be divided into three periods: early-or mid uninucleate, late-uninucleate and binucleate. A significant correlation could be found between development stages of microspore and buds morphology. Anthers at different developmental stages judged... |
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| Keywords: | Medicago truncatula buds microspore development anther dedifferentiation |
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